CRISPR

CRISPR1-ccdc32

ID
ZDB-CRISPR-210113-4
Name
CRISPR1-ccdc32
Previous Names
None
Target
Sequence
5' - GCTAAAGTTAGCAGCTCTGG - 3'
ZFIN BLAST NCBI BLAST Ensembl UCSC BLAT
Disclaimer
Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.
Note
The PAM site was "GGG" at the 3' end.
Genome Resources
None
Target Location
Constructs
No data available
Genomic Features
No data available
Expression
Gene expression in Wild Types + CRISPR1-ccdc32
Expressed Gene Anatomy Figures
spaw Fig. 3 from Harel et al., 2020
Phenotype
Phenotype resulting from CRISPR1-ccdc32
Phenotype Fish Figures
ceratohyal cartilage increased angle to ceratohyal cartilage, abnormal zf195Tg + CRISPR1-ccdc32 Fig. 2 from Harel et al., 2020
cerebellum decreased area, abnormal WT + CRISPR1-ccdc32 Fig. 2 from Harel et al., 2020
embryonic heart tube left/right pattern formation disrupted, abnormal WT + CRISPR1-ccdc32 Fig. 3 from Harel et al., 2020
head decreased size, abnormal WT + CRISPR1-ccdc32 Fig. 2 from Harel et al., 2020
heart looping disrupted, abnormal WT + CRISPR1-ccdc32 Fig. 3 from Harel et al., 2020
Kupffer's vesicle cilium decreased amount, abnormal WT + CRISPR1-ccdc32 Fig. 4 from Harel et al., 2020
Kupffer's vesicle cilium decreased length, abnormal WT + CRISPR1-ccdc32 Fig. 4 from Harel et al., 2020
lateral plate mesoderm spaw expression mislocalised, abnormal WT + CRISPR1-ccdc32 Fig. 3 from Harel et al., 2020
lateral plate mesoderm spaw expression spatial pattern, abnormal WT + CRISPR1-ccdc32 Fig. 3 from Harel et al., 2020
left/right axis specification disrupted, abnormal WT + CRISPR1-ccdc32 Fig. 3 from Harel et al., 2020
Phenotype of all Fish created by or utilizing CRISPR1-ccdc32
Phenotype Fish Conditions Figures
Kupffer's vesicle cilium decreased length, abnormal WT + CRISPR1-ccdc32 control Fig. 4 from Harel et al., 2020
Kupffer's vesicle cilium decreased amount, abnormal WT + CRISPR1-ccdc32 control Fig. 4 from Harel et al., 2020
left/right axis specification disrupted, abnormal WT + CRISPR1-ccdc32 control Fig. 3 from Harel et al., 2020
heart looping disrupted, abnormal WT + CRISPR1-ccdc32 control Fig. 3 from Harel et al., 2020
lateral plate mesoderm spaw expression spatial pattern, abnormal WT + CRISPR1-ccdc32 control Fig. 3 from Harel et al., 2020
lateral plate mesoderm spaw expression mislocalised, abnormal WT + CRISPR1-ccdc32 control Fig. 3 from Harel et al., 2020
embryonic heart tube left/right pattern formation disrupted, abnormal WT + CRISPR1-ccdc32 control Fig. 3 from Harel et al., 2020
head decreased size, abnormal WT + CRISPR1-ccdc32 control Fig. 2 from Harel et al., 2020
cerebellum decreased area, abnormal WT + CRISPR1-ccdc32 control Fig. 2 from Harel et al., 2020
ceratohyal cartilage increased angle to ceratohyal cartilage, abnormal zf195Tg + CRISPR1-ccdc32 control Fig. 2 from Harel et al., 2020
Citations
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