CRISPR

CRISPR1-atp7a

ID

ZDB-CRISPR-200520-1

Name

CRISPR1-atp7a

Previous Names

None

Target

atp7a (1)

Sequence

5' - GGCCGGATCGGAGGCCTGCC - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The PAM site was AGG at the 3' end.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
hza4	atp7a

Expression

Gene expression in Wild Types + CRISPR1-atp7a

No data available

Phenotype

Phenotype resulting from CRISPR1-atp7a

No data available

Phenotype of all Fish created by or utilizing CRISPR1-atp7a

Phenotype	Fish	Conditions	Figures
intestine hspa5 expression increased amount, abnormal	atp7a^hza4/hza4	control	Fig. 6 from Zhao et al., 2019
intestinal epithelial cell mitochondrial inner membrane swollen, abnormal	atp7a^hza4/hza4	control	Fig. 6 from Zhao et al., 2019
whole organism cox4i2 expression increased amount, abnormal	atp7a^hza4/hza4	control	Fig. 7 from Zhao et al., 2020
whole organism eif2ak3 expression increased amount, abnormal	atp7a^hza4/hza4	control	Fig. 7 from Zhao et al., 2020
whole organism fabp2 expression decreased amount, abnormal	atp7a^hza4/hza4	chemical treatment: copper atom	Fig. 6 from Zhao et al., 2019
whole organism hmox2a expression increased amount, abnormal	atp7a^hza4/hza4	control	Fig. 7 from Zhao et al., 2020
intestine slc15a1b expression decreased amount, abnormal	atp7a^hza4/hza4	control	Fig. 6 from Zhao et al., 2019
intestine fabp2 expression decreased amount, abnormal	atp7a^hza4/hza4	chemical treatment: copper atom	Fig. 6 from Zhao et al., 2019
retina opn1sw1 expression decreased amount, abnormal	atp7a^hza4/hza4	chemical treatment: copper atom	Fig. 7 from Zhao et al., 2020
retina opn1sw1 expression decreased amount, abnormal	atp7a^hza4/hza4	chemical treatment: copper(2+)	Fig. 7 from Zhao et al., 2020
whole organism eif2ak3 expression amount, ameliorated	atp7a^hza4/hza4	chemical treatment: copper(2+)	Fig. 7 from Zhao et al., 2020
whole organism ern1 expression increased amount, abnormal	atp7a^hza4/hza4	control	Fig. 7 from Zhao et al., 2020
whole organism cox4i2 expression amount, ameliorated	atp7a^hza4/hza4	chemical treatment: copper(2+)	Fig. 7 from Zhao et al., 2020
retina mitochondrion swollen, abnormal	atp7a^hza4/hza4	standard conditions	Fig. 7 from Zhao et al., 2020
whole organism cyp8b1.1 expression amount, ameliorated	atp7a^hza4/hza4	chemical treatment: copper atom	Fig. 6 from Zhao et al., 2019
whole organism ern1 expression amount, ameliorated	atp7a^hza4/hza4	chemical treatment: copper(2+)	Fig. 7 from Zhao et al., 2020
brain hspa5 expression decreased amount, abnormal	atp7a^hza4/hza4	chemical treatment: copper(2+)	Fig. 7 from Zhao et al., 2020
whole organism hmox2a expression amount, ameliorated	atp7a^hza4/hza4	chemical treatment: copper(2+)	Fig. 7 from Zhao et al., 2020
whole organism rho expression decreased amount, abnormal	atp7a^hza4/hza4	chemical treatment: copper(2+)	Fig. 7 from Zhao et al., 2020
whole organism slc15a1b expression decreased amount, abnormal	atp7a^hza4/hza4	control	Fig. 6 from Zhao et al., 2019
brain ddit3 expression decreased amount, abnormal	atp7a^hza4/hza4	chemical treatment: copper(2+)	Fig. 7 from Zhao et al., 2020
whole organism opn1sw1 expression decreased amount, abnormal	atp7a^hza4/hza4	chemical treatment: copper atom	Fig. 7 from Zhao et al., 2020
whole organism znrf2a expression amount, ameliorated	atp7a^hza4/hza4	chemical treatment: copper(2+)	Fig. 7 from Zhao et al., 2020
whole organism cyp3a65 expression amount, ameliorated	atp7a^hza4/hza4	chemical treatment: copper atom	Fig. 6 from Zhao et al., 2019
whole organism opn1sw2 expression decreased amount, abnormal	atp7a^hza4/hza4	chemical treatment: copper(2+)	Fig. 7 from Zhao et al., 2020
intestine fabp2 expression decreased amount, abnormal	atp7a^hza4/hza4	control	Fig. 6 from Zhao et al., 2019
brain hspa5 expression decreased amount, abnormal	atp7a^hza4/hza4	chemical treatment: copper atom	Fig. 7 from Zhao et al., 2020
whole organism loxa expression increased amount, abnormal	atp7a^hza4/hza4	control	Fig. 7 from Zhao et al., 2020
whole organism opn1sw2 expression decreased amount, abnormal	atp7a^hza4/hza4	chemical treatment: copper atom	Fig. 7 from Zhao et al., 2020
whole organism ern1 expression amount, ameliorated	atp7a^hza4/hza4	chemical treatment: copper atom	Fig. 7 from Zhao et al., 2020
whole organism eif2ak3 expression amount, ameliorated	atp7a^hza4/hza4	chemical treatment: copper atom	Fig. 7 from Zhao et al., 2020
whole organism opn1sw1 expression decreased amount, abnormal	atp7a^hza4/hza4	chemical treatment: copper(2+)	Fig. 7 from Zhao et al., 2020
whole organism slc15a1b expression decreased amount, abnormal	atp7a^hza4/hza4	chemical treatment: copper atom	Fig. 6 from Zhao et al., 2019
intestinal epithelial cell endoplasmic reticulum structure, exacerbated	atp7a^hza4/hza4	chemical treatment: copper(2+)	Fig. 6 from Zhao et al., 2019
whole organism rho expression decreased amount, abnormal	atp7a^hza4/hza4	chemical treatment: copper atom	Fig. 7 from Zhao et al., 2020
intestine hspa5 expression amount, ameliorated	atp7a^hza4/hza4	chemical treatment: copper atom	Fig. 6 from Zhao et al., 2019
intestinal epithelial cell endoplasmic reticulum structure, exacerbated	atp7a^hza4/hza4	chemical treatment: copper atom	Fig. 6 from Zhao et al., 2019
whole organism znrf2a expression increased amount, abnormal	atp7a^hza4/hza4	control	Fig. 7 from Zhao et al., 2020
whole organism atf6 expression increased amount, abnormal	atp7a^hza4/hza4	control	Fig. 7 from Zhao et al., 2020
whole organism hmox2a expression amount, ameliorated	atp7a^hza4/hza4	chemical treatment: copper atom	Fig. 7 from Zhao et al., 2020
brain ddit3 expression decreased amount, abnormal	atp7a^hza4/hza4	chemical treatment: copper atom	Fig. 7 from Zhao et al., 2020
whole organism cyp3a65 expression decreased amount, abnormal	atp7a^hza4/hza4	control	Fig. 6 from Zhao et al., 2019
whole organism loxa expression amount, ameliorated	atp7a^hza4/hza4	chemical treatment: copper atom	Fig. 7 from Zhao et al., 2020
retina mitochondrion swollen, abnormal	atp7a^hza4/hza4	chemical treatment: copper atom	Fig. 7 from Zhao et al., 2020
intestinal epithelial cell mitochondrial inner membrane swollen, exacerbated	atp7a^hza4/hza4	chemical treatment: copper(2+)	Fig. 6 from Zhao et al., 2019
whole organism znrf2a expression amount, ameliorated	atp7a^hza4/hza4	chemical treatment: copper atom	Fig. 7 from Zhao et al., 2020
retina opn1sw2 expression decreased amount, abnormal	atp7a^hza4/hza4	chemical treatment: copper atom	Fig. S8 from Zhao et al., 2020
retina mitochondrion swollen, abnormal	atp7a^hza4/hza4	chemical treatment: copper(2+)	Fig. 7 from Zhao et al., 2020
whole organism cox4i2 expression amount, ameliorated	atp7a^hza4/hza4	chemical treatment: copper atom	Fig. 7 from Zhao et al., 2020
whole organism cyp8b1.1 expression decreased amount, abnormal	atp7a^hza4/hza4	control	Fig. 6 from Zhao et al., 2019
whole organism atf6 expression amount, ameliorated	atp7a^hza4/hza4	chemical treatment: copper atom	Fig. 7 from Zhao et al., 2020
intestinal epithelial cell mitochondrial inner membrane swollen, exacerbated	atp7a^hza4/hza4	chemical treatment: copper atom	Fig. 6 from Zhao et al., 2019
whole organism fabp2 expression decreased amount, abnormal	atp7a^hza4/hza4	control	Fig. 6 from Zhao et al., 2019
whole organism atf6 expression amount, ameliorated	atp7a^hza4/hza4	chemical treatment: copper(2+)	Fig. 7 from Zhao et al., 2020
intestinal epithelial cell endoplasmic reticulum loose, abnormal	atp7a^hza4/hza4	control	Fig. 6 from Zhao et al., 2019
whole organism loxa expression amount, ameliorated	atp7a^hza4/hza4	chemical treatment: copper(2+)	Fig. 7 from Zhao et al., 2020
intestine slc15a1b expression decreased amount, abnormal	atp7a^hza4/hza4	chemical treatment: copper atom	Fig. 6 from Zhao et al., 2019

Citations