CRISPR

CRISPR1-ikzf1

ID

ZDB-CRISPR-200512-4

Name

CRISPR1-ikzf1

Previous Names

None

Target

ikzf1 (1)

Sequence

5' - GGCGGAGAGGATCAAGATG - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The PAM site was "AGG" at the 3' end.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
zf3330	ikzf1

Expression

Gene expression in Wild Types + CRISPR1-ikzf1

No data available

Phenotype

Phenotype resulting from CRISPR1-ikzf1

No data available

Phenotype of all Fish created by or utilizing CRISPR1-ikzf1

Phenotype	Fish	Conditions	Figures
caudal hematopoietic tissue irf4a expression decreased amount, abnormal	ikzf1^{zf3330/zf3330}	standard conditions	Fig. 6 from Huang et al., 2019
thymus rag1 expression decreased amount, abnormal	ikzf1^{zf3330/zf3330}	standard conditions	Fig. 5 from Huang et al., 2019
thymus ccr9b expression absent, abnormal	ikzf1^{zf3330/zf3330}	standard conditions	Fig. 1 from Huang et al., 2019
thymus ccr9a expression absent, abnormal	ikzf1^{zf3330/zf3330}	standard conditions	Fig. 1 from Huang et al., 2019
thymus myb expression decreased amount, abnormal	ikzf1^{zf3330/zf3330}	standard conditions	Fig. 2, Fig. 5 from Huang et al., 2019
hematopoietic stem cell cell population proliferation decreased occurrence, abnormal	ikzf1^{zf3330/zf3330}	standard conditions	Fig. 3 from Huang et al., 2019
thymus lck expression absent, abnormal	ikzf1^{zf3330/zf3330}	standard conditions	Fig. 1, Fig. 7 from Huang et al., 2019
thymus rag1 expression absent, abnormal	ikzf1^{zf3330/zf3330}	standard conditions	Fig. 1, Fig. 7 from Huang et al., 2019
hematopoietic stem cell positive regulation of cell migration disrupted, abnormal	ikzf1^{zf3330/zf3330}	standard conditions	Fig. 5 from Huang et al., 2019
caudal hematopoietic tissue myb expression decreased amount, abnormal	ikzf1^{zf3330/zf3330}	standard conditions	Fig. 3 from Huang et al., 2019
thymus T cell decreased amount, abnormal	ikzf1^{zf3330/zf3330}; cq28Tg; nz117Tg	standard conditions	Fig. 2 from Huang et al., 2019
thymus hematopoietic stem cell decreased amount, abnormal	ikzf1^{zf3330/zf3330}; cq28Tg; nz117Tg	standard conditions	Fig. 2 from Huang et al., 2019
thymus hematopoietic stem cell decreased amount, abnormal	ikzf1^{zf3330/zf3330}; la2Tg	standard conditions	Fig. 5 from Huang et al., 2019
thymus hematopoietic stem cell normal amount, ameliorated	ikzf1^{zf3330/zf3330}; la2Tg; zf3328Tg	standard conditions	Fig. 5 from Huang et al., 2019
thymus rag1 expression decreased amount, abnormal	ikzf1^{zf3330/zf3330}; zf3328Tg	standard conditions	Fig. 5 from Huang et al., 2019
thymus myb expression amount, ameliorated	ikzf1^{zf3330/zf3330}; zf3328Tg	standard conditions	Fig. 5 from Huang et al., 2019
hematopoietic stem cell cell migration normal occurrence, ameliorated	ikzf1^{zf3330/zf3330}; zf3328Tg	standard conditions	Fig. 5 from Huang et al., 2019
thymus lck expression amount, ameliorated	ikzf1^{zf3330/zf3330}; zf3329Tg	standard conditions	Fig. 7 from Huang et al., 2019
thymus rag1 expression amount, ameliorated	ikzf1^{zf3330/zf3330}; zf3329Tg	standard conditions	Fig. 7 from Huang et al., 2019
thymus rag1 expression amount, ameliorated	ccr9a^{zf3332/zf3332}; ikzf1^{zf3330/zf3330}; zf3329Tg	standard conditions	Fig. 7 from Huang et al., 2019
thymus lck expression amount, ameliorated	ccr9a^{zf3332/zf3332}; ikzf1^{zf3330/zf3330}; zf3329Tg	standard conditions	Fig. 7 from Huang et al., 2019
thymus rag1 expression amount, ameliorated	ikzf1^{zf3330/zf3330}; zf3328Tg; zf3329Tg	standard conditions	Fig. 7 from Huang et al., 2019
thymus lck expression amount, ameliorated	ikzf1^{zf3330/zf3330}; zf3328Tg; zf3329Tg	standard conditions	Fig. 7 from Huang et al., 2019

Citations