CRISPR

CRISPR1-scxa

ID

ZDB-CRISPR-191227-3

Name

CRISPR1-scxa

Previous Names

None

Target

scxa (1)

Sequence

5' - GGGGGTGGCGGACGGCTGAT - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

None

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
kg141	scxa
kg170	scxa

Expression

Gene expression in Wild Types + CRISPR1-scxa

No data available

Phenotype

Phenotype resulting from CRISPR1-scxa

No data available

Phenotype of all Fish created by or utilizing CRISPR1-scxa

Phenotype	Fish	Conditions	Figures
dentary decreased mass density, abnormal	scxa^kg170/kg170 (AB)	standard conditions	Fig. 4 from Kague et al., 2019
ventral intermandibularis posterior tendon malformed, abnormal	scxa^kg170/kg170 (AB)	standard conditions	Fig. 2 from Kague et al., 2019
rib of vertebra 5 absent, abnormal	scxa^kg170/kg170 (AB)	standard conditions	Fig. 5 from Kague et al., 2019
Meckel's cartilage ligament tnmd expression decreased amount, abnormal	scxa^kg170/kg170 (AB)	standard conditions	Fig. 2 from Kague et al., 2019
vertical myoseptum muscle tendon junction tnmd expression decreased amount, abnormal	scxa^kg170/kg170 (AB)	standard conditions	Fig. 6 from Kague et al., 2019
female organism decreased weight, abnormal	scxa^kg170/kg170 (AB)	standard conditions	Fig. 3 from Kague et al., 2019
rib of vertebra 6 absent, abnormal	scxa^kg170/kg170 (AB)	standard conditions	Fig. 5 from Kague et al., 2019
dentary composition, abnormal	scxa^kg170/kg170 (AB)	standard conditions	Fig. 4 from Kague et al., 2019
rib bone mineralization decreased process quality, abnormal	scxa^kg170/kg170 (AB)	standard conditions	Fig. 4 , Fig. 5 from Kague et al., 2019
swimming decreased process quality, abnormal	scxa^kg170/kg170 (AB)	standard conditions	Fig. 3 from Kague et al., 2019
male organism decreased weight, abnormal	scxa^kg170/kg170 (AB)	standard conditions	Fig. 3 from Kague et al., 2019
splanchnocranium tendon development decreased process quality, abnormal	scxa^kg170/kg170 (AB)	standard conditions	Fig. 2 from Kague et al., 2019
adductor mandibulae skeletal muscle cell mislocalised, abnormal	scxa^kg170/kg170 (AB)	standard conditions	Fig. 2 from Kague et al., 2019
interhyoideus tendon malformed, abnormal	scxa^kg170/kg170 (AB)	standard conditions	Fig. 2 from Kague et al., 2019
palatoquadrate cartilage ligament tnmd expression decreased amount, abnormal	scxa^kg170/kg170 (AB)	standard conditions	Fig. 2 from Kague et al., 2019
intervertebral ligament malformed, abnormal	scxa^kg170/kg170 (AB)	standard conditions	Fig. 4 from Kague et al., 2019
interhyoideus skeletal muscle cell mislocalised, abnormal	scxa^kg170/kg170 (AB)	standard conditions	Fig. 2 from Kague et al., 2019
myotome decreased volume, abnormal	scxa^kg170/kg170 (AB)	standard conditions	Fig. 3 from Kague et al., 2019
skeletal system lacks parts or has fewer parts of type rib, abnormal	scxa^kg170/kg170 (AB)	standard conditions	Fig. 5 from Kague et al., 2019
ceratohyal cartilage tendon malformed, abnormal	scxa^kg170/kg170 (AB)	standard conditions	Fig. 2 from Kague et al., 2019
skeletal system lacks all parts of type rib, abnormal	scxa^kg170/kg170 (AB)	standard conditions	Fig. 3 , Fig. 4 from Kague et al., 2019
sternohyoid tendon tnmd expression decreased amount, abnormal	scxa^kg170/kg170 (AB)	standard conditions	Fig. 2 from Kague et al., 2019
rib bone morphogenesis decreased process quality, abnormal	scxa^kg170/kg170 (AB)	standard conditions	Fig. 4 , Fig. 5 from Kague et al., 2019
splanchnocranium skeletal muscle tissue development decreased process quality, abnormal	scxa^kg170/kg170 (AB)	standard conditions	Fig. 2 from Kague et al., 2019
hyohyoideus skeletal muscle cell mislocalised, abnormal	scxa^kg170/kg170 (AB)	standard conditions	Fig. 2 from Kague et al., 2019
sternohyoid tendon composition, abnormal	scxa^kg170/kg170; hu5910Tg (AB)	standard conditions	Fig. 2 from Kague et al., 2019
splanchnocranium tendon development decreased process quality, abnormal	scxa^kg170/kg170; hu5910Tg (AB)	standard conditions	Fig. 2 from Kague et al., 2019
splanchnocranium skeletal muscle tissue development decreased process quality, abnormal	scxa^kg170/kg170; hu5910Tg (AB)	standard conditions	Fig. 2 from Kague et al., 2019
tendon collagen fibril organization decreased process quality, abnormal	scxa^kg170/kg170; hu5910Tg (AB)	standard conditions	Fig. 2 from Kague et al., 2019
interhyoideus skeletal muscle cell increased length, abnormal	scxa^kg170/kg170; scxb^kg107/kg107 (AB)	standard conditions	Fig. 7 from Kague et al., 2019
splanchnocranium skeletal muscle tissue development decreased process quality, exacerbated	scxa^kg170/kg170; scxb^kg107/kg107 (AB)	standard conditions	Fig. 7 from Kague et al., 2019
ventral intermandibularis posterior skeletal muscle cell mislocalised, abnormal	scxa^kg170/kg170; scxb^kg107/kg107 (AB)	standard conditions	Fig. 7 from Kague et al., 2019
interhyoideus tendon Ab4-thbs4b labeling decreased amount, abnormal	scxa^kg170/kg170; scxb^kg107/kg107 (AB)	standard conditions	Fig. 7 from Kague et al., 2019
splanchnocranium tendon development decreased process quality, exacerbated	scxa^kg170/kg170; scxb^kg107/kg107 (AB)	standard conditions	Fig. 7 from Kague et al., 2019
ventral intermandibularis posterior skeletal muscle cell increased length, abnormal	scxa^kg170/kg170; scxb^kg107/kg107 (AB)	standard conditions	Fig. 7 from Kague et al., 2019
ventral intermandibularis posterior tendon Ab4-thbs4b labeling decreased amount, abnormal	scxa^kg170/kg170; scxb^kg107/kg107 (AB)	standard conditions	Fig. 7 from Kague et al., 2019
ventral mandibular arch paralysed, abnormal	scxa^kg170/kg170; scxb^kg107/kg107 (AB)	standard conditions	Fig. 7 from Kague et al., 2019
adductor mandibulae tendon Ab4-thbs4b labeling decreased amount, abnormal	scxa^kg170/kg170; scxb^kg107/kg107 (AB)	standard conditions	Fig. 7 from Kague et al., 2019
interhyoideus skeletal muscle cell mislocalised, abnormal	scxa^kg170/kg170; scxb^kg107/kg107 (AB)	standard conditions	Fig. 7 from Kague et al., 2019
Meckel's cartilage chondrocyte undifferentiated, abnormal	scxa^kg170/kg170; scxb^kg107/kg107; hu5910Tg (AB)	standard conditions	Fig. 7 from Kague et al., 2019
Meckel's cartilage chondrocyte differentiation decreased process quality, abnormal	scxa^kg170/kg170; scxb^kg107/kg107; hu5910Tg (AB)	standard conditions	Fig. 7 from Kague et al., 2019
Meckel's cartilage chondrocyte immature, abnormal	scxa^kg170/kg170; scxb^kg107/kg107; hu5910Tg (AB)	standard conditions	Fig. 7 from Kague et al., 2019
Meckel's cartilage drooping, abnormal	scxa^kg170/kg170; scxb^kg107/kg107; hu5910Tg (AB)	standard conditions	Fig. 7 from Kague et al., 2019
Meckel's cartilage malformed, abnormal	scxa^kg170/kg170; scxb^kg107/kg107; hu5910Tg (AB)	standard conditions	Fig. 7 from Kague et al., 2019
Meckel's cartilage chondrocyte obtuse, abnormal	scxa^kg170/kg170; scxb^kg107/kg107; hu5910Tg (AB)	standard conditions	Fig. 7 from Kague et al., 2019
ventral mandibular arch paralysed, abnormal	scxa^kg170/kg170; scxb^kg107/kg107; hu5910Tg (AB)	standard conditions	Fig. 7 from Kague et al., 2019
whole organism lethal (sensu genetics), abnormal	scxa^kg170/kg170; scxb^kg107/kg107; hu5910Tg (AB)	standard conditions	Fig. 7 from Kague et al., 2019

Citations