CRISPR

CRISPR1-gtpbp3

ID

ZDB-CRISPR-191106-1

Name

CRISPR1-gtpbp3

Previous Names

None

Target

gtpbp3 (1)

Sequence

5' - GGTGGTCCGGGTCAGCGGTC - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The PAM sequence is CGG at the 3' end.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
zf2239	gtpbp3

Expression

Gene expression in Wild Types + CRISPR1-gtpbp3

No data available

Phenotype

Phenotype resulting from CRISPR1-gtpbp3

No data available

Phenotype of all Fish created by or utilizing CRISPR1-gtpbp3

Phenotype	Fish	Conditions	Figures
whole organism mitochondrion mto1 expression decreased amount, abnormal	gtpbp3^{zf2239/zf2239} (AB)	standard conditions	Fig. 5 from Chen et al., 2019
whole organism respiratory chain complex II (succinate dehydrogenase) decreased amount, abnormal	gtpbp3^{zf2239/zf2239} (AB)	standard conditions	Fig. 6 from Chen et al., 2019
whole organism respiratory chain complex I decreased efficiency, abnormal	gtpbp3^{zf2239/zf2239} (AB)	standard conditions	Fig. 7 from Chen et al., 2019
whole organism mitochondrion mt-co2 expression decreased amount, abnormal	gtpbp3^{zf2239/zf2239} (AB)	standard conditions	Fig. 5 from Chen et al., 2019
whole organism mitochondrion trmu expression decreased amount, abnormal	gtpbp3^{zf2239/zf2239} (AB)	standard conditions	Fig. 5 from Chen et al., 2019
whole organism mitochondrion mt-nd5 expression decreased amount, abnormal	gtpbp3^{zf2239/zf2239} (AB)	standard conditions	Fig. 5 from Chen et al., 2019
heart nppb expression increased amount, abnormal	gtpbp3^{zf2239/zf2239} (AB)	standard conditions	Fig. 9 from Chen et al., 2019
mitochondrial electron transport, cytochrome c to oxygen decreased occurrence, abnormal	gtpbp3^{zf2239/zf2239} (AB)	standard conditions	Fig. 10 from Chen et al., 2019
whole organism respiratory chain complex IV decreased efficiency, abnormal	gtpbp3^{zf2239/zf2239} (AB)	standard conditions	Fig. 7 from Chen et al., 2019
whole organism proton-transporting ATP synthase complex decreased efficiency, abnormal	gtpbp3^{zf2239/zf2239} (AB)	standard conditions	Fig. 7 from Chen et al., 2019
whole organism mitochondrion yars2 expression decreased amount, abnormal	gtpbp3^{zf2239/zf2239} (AB)	standard conditions	Fig. 5 from Chen et al., 2019
whole organism obsolete mitochondrial respiratory chain complex II, succinate dehydrogenase complex (ubiquinone) decreased efficiency, abnormal	gtpbp3^{zf2239/zf2239} (AB)	standard conditions	Fig. 7 from Chen et al., 2019
cardiac muscle disorganized, abnormal	gtpbp3^{zf2239/zf2239} (AB)	standard conditions	Fig. 9 from Chen et al., 2019
whole organism respiratory chain complex IV decreased amount, abnormal	gtpbp3^{zf2239/zf2239} (AB)	standard conditions	Fig. 6 from Chen et al., 2019
whole organism mitochondrion kars1 expression decreased amount, abnormal	gtpbp3^{zf2239/zf2239} (AB)	standard conditions	Fig. 5 from Chen et al., 2019
whole organism mitochondrion tufm expression decreased amount, abnormal	gtpbp3^{zf2239/zf2239} (AB)	standard conditions	Fig. 5 from Chen et al., 2019
cardiac muscle hypertrophic, abnormal	gtpbp3^{zf2239/zf2239} (AB)	standard conditions	Fig. 9 from Chen et al., 2019
mitochondrial lysyl-tRNA aminoacylation increased occurrence, abnormal	gtpbp3^{zf2239/zf2239} (AB)	standard conditions	Fig. 4 from Chen et al., 2019
mitochondrial methionyl-tRNA aminoacylation increased occurrence, abnormal	gtpbp3^{zf2239/zf2239} (AB)	standard conditions	Fig. 4 from Chen et al., 2019
mitochondrial tryptophanyl-tRNA aminoacylation increased occurrence, abnormal	gtpbp3^{zf2239/zf2239} (AB)	standard conditions	Fig. 4 from Chen et al., 2019
whole organism clpp expression increased amount, abnormal	gtpbp3^{zf2239/zf2239} (AB)	standard conditions	Fig. 6 from Chen et al., 2019
mitochondrial electron transport, succinate to ubiquinone decreased occurrence, abnormal	gtpbp3^{zf2239/zf2239} (AB)	standard conditions	Fig. 10 from Chen et al., 2019
whole organism afg3l2 expression increased amount, abnormal	gtpbp3^{zf2239/zf2239} (AB)	standard conditions	Fig. 6 from Chen et al., 2019
whole organism mitochondrion atp5f1c expression decreased amount, abnormal	gtpbp3^{zf2239/zf2239} (AB)	standard conditions	Fig. 5 from Chen et al., 2019
whole organism mitochondrion mt-nd6 expression decreased amount, abnormal	gtpbp3^{zf2239/zf2239} (AB)	standard conditions	Fig. 5 from Chen et al., 2019
cardiac muscle cell increased size, abnormal	gtpbp3^{zf2239/zf2239} (AB)	standard conditions	Fig. 9 from Chen et al., 2019
heart mitochondrial crista shape, abnormal	gtpbp3^{zf2239/zf2239} (AB)	standard conditions	Fig. 10 from Chen et al., 2019
leucyl-tRNA aminoacylation increased occurrence, abnormal	gtpbp3^{zf2239/zf2239} (AB)	standard conditions	Fig. 4 from Chen et al., 2019
whole organism respiratory chain complex II (succinate dehydrogenase) decreased efficiency, abnormal	gtpbp3^{zf2239/zf2239} (AB)	standard conditions	Fig. 7 from Chen et al., 2019
mitochondrial alanyl-tRNA aminoacylation increased occurrence, abnormal	gtpbp3^{zf2239/zf2239} (AB)	standard conditions	Fig. 4 from Chen et al., 2019
whole organism mitochondrion tfam expression decreased amount, abnormal	gtpbp3^{zf2239/zf2239} (AB)	standard conditions	Fig. 5 from Chen et al., 2019
whole organism respiratory chain complex I decreased amount, abnormal	gtpbp3^{zf2239/zf2239} (AB)	standard conditions	Fig. 6 from Chen et al., 2019
cardiac muscle cell nucleus shape, abnormal	gtpbp3^{zf2239/zf2239} (AB)	standard conditions	Fig. 9 from Chen et al., 2019
heart mitochondrion swollen, abnormal	gtpbp3^{zf2239/zf2239} (AB)	standard conditions	Fig. 10 from Chen et al., 2019
heart nppa expression increased amount, abnormal	gtpbp3^{zf2239/zf2239} (AB)	standard conditions	Fig. 9 from Chen et al., 2019
whole organism mitochondrion mt-cyb expression decreased amount, abnormal	gtpbp3^{zf2239/zf2239} (AB)	standard conditions	Fig. 5 from Chen et al., 2019
whole organism mitochondrion sdha expression decreased amount, abnormal	gtpbp3^{zf2239/zf2239} (AB)	standard conditions	Fig. 5 from Chen et al., 2019
whole organism obsolete mitochondrial respiratory chain complex IV decreased efficiency, abnormal	gtpbp3^{zf2239/zf2239} (AB)	standard conditions	Fig. 7 from Chen et al., 2019
cardiac muscle cell I band increased length, abnormal	gtpbp3^{zf2239/zf2239} (AB)	standard conditions	Fig. 9 from Chen et al., 2019
mitochondrial tyrosyl-tRNA aminoacylation increased occurrence, abnormal	gtpbp3^{zf2239/zf2239} (AB)	standard conditions	Fig. 4 from Chen et al., 2019
heart looping disrupted, abnormal	gtpbp3^{zf2239/zf2239}; twu34Tg	standard conditions	Fig. 8 from Chen et al., 2019
heart contraction disrupted, abnormal	gtpbp3^{zf2239/zf2239}; twu34Tg	standard conditions	Fig. 8 from Chen et al., 2019

Citations