CRISPR

CRISPR2-vdra

ID

ZDB-CRISPR-190906-13

Name

CRISPR2-vdra

Previous Names

None

Target

vdra (1)

Sequence

5' - GGCGTTGAAGTGGAATCCGG - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

None

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
pd307	vdra

Expression

Gene expression in Wild Types + CRISPR2-vdra

No data available

Phenotype

Phenotype resulting from CRISPR2-vdra

No data available

Phenotype of all Fish created by or utilizing CRISPR2-vdra

Phenotype	Fish	Conditions	Figures
liver fatty acid homeostasis decreased process quality, abnormal	vdra^pd307/+; vdrb^pd308/pd308	standard conditions	Fig. 1 from Freeburg et al., 2024
whole organism increased mass, abnormal	vdra^pd307/+; vdrb^pd308/pd308	high fat	Fig. 1 from Freeburg et al., 2024
abdominal musculature fatty acid homeostasis decreased process quality, abnormal	vdra^pd307/+; vdrb^pd308/pd308	high fat	Fig. 1 from Freeburg et al., 2024
whole organism decreased mass, abnormal	vdra^pd307/pd307; vdrb^pd308/pd308	standard conditions	Fig. 1 from Freeburg et al., 2024
abdominal musculature fatty acid homeostasis decreased process quality, abnormal	vdra^pd307/pd307; vdrb^pd308/pd308	standard conditions	Fig. 1 from Freeburg et al., 2024
whole organism decreased length, abnormal	vdra^pd307/pd307; vdrb^pd308/pd308	control	Fig. 2 from Han et al., 2019
liver fatty acid homeostasis decreased process quality, exacerbated	vdra^pd307/pd307; vdrb^pd308/pd308	standard conditions	Fig. 1 from Freeburg et al., 2024
whole organism increased mass, ameliorated	vdra^pd307/pd307; vdrb^pd308/pd308	high fat	Fig. 1 from Freeburg et al., 2024
abdominal musculature fatty acid homeostasis decreased process quality, exacerbated	vdra^pd307/pd307; vdrb^pd308/pd308	high fat	Fig. 1 from Freeburg et al., 2024
cardiac ventricle cardiac muscle cell proliferation occurrence, ameliorated	vdra^pd307/pd307; vdrb^pd308/pd308	chemical treatment by injection: alfacalcidol	Fig. 1 from Han et al., 2019
growth rate, ameliorated	vdra^pd307/pd307; vdrb^pd308/pd308	chemical treatment by environment: alfacalcidol	Fig. 2 from Han et al., 2019
whole organism length, ameliorated	vdra^pd307/pd307; vdrb^pd308/pd308	chemical treatment by environment: alfacalcidol	Fig. 2 from Han et al., 2019
heart GFP expression absent, abnormal	vdra^pd307/pd307; vdrb^pd308/pd308; cyp24a1^pd272Tg/+	chemical treatment by environment: alfacalcidol	Fig. 2 from Han et al., 2019
whole organism GFP expression absent, abnormal	vdra^pd307/pd307; vdrb^pd308/pd308; cyp24a1^pd272Tg/+	chemical treatment by environment: alfacalcidol	Fig. 2 from Han et al., 2019

Citations