CRISPR

CRISPR2-spaw

ID

ZDB-CRISPR-190422-2

Name

CRISPR2-spaw

Previous Names

None

Target

spaw (1)

Sequence

5' - GTACCTTGGATAAAACTGGC - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

None

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
a205	spaw

Expression

Gene expression in Wild Types + CRISPR2-spaw

No data available

Phenotype

Phenotype resulting from CRISPR2-spaw

No data available

Phenotype of all Fish created by or utilizing CRISPR2-spaw

Phenotype	Fish	Conditions	Figures
Kupffer's vesicle anatomical region spaw expression decreased amount, abnormal	spaw^a205/a205 (AB/TL)	standard conditions	Fig. 1 from Montague et al., 2018
heart jogging process quality, abnormal	spaw^a205/a205 (AB/TL)	standard conditions	Fig. 1 from Montague et al., 2018
heart looping decreased occurrence, abnormal	spaw^a205/a205 (AB/TL)	standard conditions	Fig. 1 from Montague et al., 2018
notochord lft1 expression absent, abnormal	spaw^a205/a205 (AB/TL)	standard conditions	Fig. 1 from Montague et al., 2018
lateral plate mesoderm spaw expression absent, abnormal	spaw^a205/a205 (AB/TL)	standard conditions	Fig. 3 from Montague et al., 2018
heart looping process quality, abnormal	spaw^a205/a205 (AB/TL)	standard conditions	Fig. 1 from Montague et al., 2018
heart jogging decreased occurrence, abnormal	spaw^a205/a205 (AB/TL)	standard conditions	Fig. 1 from Montague et al., 2018
heart primordium lft2 expression absent, abnormal	spaw^a205/a205 (AB/TL)	standard conditions	Fig. 1 from Montague et al., 2018
whole organism medial region lft1 expression absent, abnormal	spaw^a205/a205 (AB/TL)	standard conditions	Fig. 1 from Montague et al., 2018
heart jogging process quality, abnormal	dand5^a204/a204; spaw^a205/a205 (AB/TL)	standard conditions	Fig. 1 from Montague et al., 2018
notochord lft1 expression absent, abnormal	dand5^a204/a204; spaw^a205/a205 (AB/TL)	standard conditions	Fig. 1 from Montague et al., 2018
heart looping decreased occurrence, abnormal	dand5^a204/a204; spaw^a205/a205 (AB/TL)	standard conditions	Fig. 1 from Montague et al., 2018
lateral plate mesoderm spaw expression absent, abnormal	dand5^a204/a204; spaw^a205/a205 (AB/TL)	standard conditions	Fig. 3 from Montague et al., 2018
heart jogging decreased occurrence, abnormal	dand5^a204/a204; spaw^a205/a205 (AB/TL)	standard conditions	Fig. 1 from Montague et al., 2018
heart looping process quality, abnormal	dand5^a204/a204; spaw^a205/a205 (AB/TL)	standard conditions	Fig. 1 from Montague et al., 2018
Kupffer's vesicle anatomical region spaw expression decreased amount, abnormal	dand5^a204/a204; spaw^a205/a205 (AB/TL)	standard conditions	Fig. 1 from Montague et al., 2018
heart primordium lft2 expression absent, abnormal	dand5^a204/a204; spaw^a205/a205 (AB/TL)	standard conditions	Fig. 1 from Montague et al., 2018
whole organism medial region lft1 expression absent, abnormal	dand5^a204/a204; spaw^a205/a205 (AB/TL)	standard conditions	Fig. 1 from Montague et al., 2018
heart jogging process quality, abnormal	spaw^a205/a205; lft1^a145/a145 (AB/TL)	standard conditions	Fig. 1 from Montague et al., 2018
notochord lft1 expression absent, abnormal	spaw^a205/a205; lft1^a145/a145 (AB/TL)	standard conditions	Fig. 1 from Montague et al., 2018
heart looping decreased occurrence, abnormal	spaw^a205/a205; lft1^a145/a145 (AB/TL)	standard conditions	Fig. 1 from Montague et al., 2018
lateral plate mesoderm spaw expression absent, abnormal	spaw^a205/a205; lft1^a145/a145 (AB/TL)	standard conditions	Fig. 3 from Montague et al., 2018
heart jogging decreased occurrence, abnormal	spaw^a205/a205; lft1^a145/a145 (AB/TL)	standard conditions	Fig. 1 from Montague et al., 2018
heart looping process quality, abnormal	spaw^a205/a205; lft1^a145/a145 (AB/TL)	standard conditions	Fig. 1 from Montague et al., 2018
Kupffer's vesicle anatomical region spaw expression decreased amount, abnormal	spaw^a205/a205; lft1^a145/a145 (AB/TL)	standard conditions	Fig. 1 from Montague et al., 2018
heart primordium lft2 expression absent, abnormal	spaw^a205/a205; lft1^a145/a145 (AB/TL)	standard conditions	Fig. 1 from Montague et al., 2018
whole organism medial region lft1 expression absent, abnormal	spaw^a205/a205; lft1^a145/a145 (AB/TL)	standard conditions	Fig. 1 from Montague et al., 2018

Citations