CRISPR

CRISPR1-hey2

ID

ZDB-CRISPR-190401-1

Name

CRISPR1-hey2

Previous Names

None

Target

hey2 (1)

Sequence

5' - CCAGAAAGAAGCGGAGAG - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

None

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
hsc25	hey2
hsc27Tg	hey2

Expression

Gene expression in Wild Types + CRISPR1-hey2

No data available

Phenotype

Phenotype resulting from CRISPR1-hey2

No data available

Phenotype of all Fish created by or utilizing CRISPR1-hey2

Phenotype	Fish	Conditions	Figures
whole organism nkx2.5 expression increased amount, abnormal	hey2^hsc25/hsc25	standard conditions	Fig. 6 from Gibb et al., 2018
whole organism myl7 expression increased amount, abnormal	hey2^hsc25/hsc25	standard conditions	Fig. 1 , Fig. 2 from Gibb et al., 2018
atrium cardiac muscle cell condensed, abnormal	hey2^hsc25/hsc25	standard conditions	Fig. 1 from Gibb et al., 2018
bulbus arteriosus elongated, abnormal	hey2^hsc25/hsc25	control	Fig. 3 from Gibb et al., 2018
heart tube mef2cb expression increased distribution, abnormal	hey2^hsc25/hsc25	standard conditions	Fig. 6 from Gibb et al., 2018
anterior lateral plate mesoderm mef2cb expression increased distribution, abnormal	hey2^hsc25/hsc25	standard conditions	Fig. 6 from Gibb et al., 2018
whole organism mef2cb expression increased amount, abnormal	hey2^hsc25/hsc25	standard conditions	Fig. 6 from Gibb et al., 2018
heart tube ltbp3 expression increased amount, abnormal	hey2^hsc25/hsc25	standard conditions	Fig. 6 from Gibb et al., 2018
anterior lateral plate mesoderm hey2 expression increased amount, abnormal	hey2^hsc25/hsc25	standard conditions	Fig. 6 from Gibb et al., 2018
bulbus arteriosus cardiac muscle cell increased amount, abnormal	hey2^hsc25/hsc25	control	Fig. 3 from Gibb et al., 2018
anterior lateral plate mesoderm nkx2.5 expression increased distribution, abnormal	hey2^hsc25/hsc25	standard conditions	Fig. 6 from Gibb et al., 2018
cardiac ventricle cardiac muscle cell condensed, abnormal	hey2^hsc25/hsc25	standard conditions	Fig. 1 from Gibb et al., 2018
heart tube nkx2.5 expression increased distribution, abnormal	hey2^hsc25/hsc25	standard conditions	Fig. 6 from Gibb et al., 2018
anterior lateral plate mesoderm hey2 expression increased distribution, abnormal	hey2^hsc25/hsc25	standard conditions	Fig. 6 from Gibb et al., 2018
whole organism ltbp3 expression increased amount, abnormal	hey2^hsc25/hsc25	standard conditions	Fig. 6 from Gibb et al., 2018
whole organism hey2 expression increased amount, abnormal	hey2^hsc25/hsc25	standard conditions	Fig. 6 from Gibb et al., 2018
primitive heart tube shape, abnormal	hey2^hsc25/hsc25	standard conditions	Fig. 2 from Gibb et al., 2018
heart tube ltbp3 expression increased distribution, abnormal	hey2^hsc25/hsc25	standard conditions	Fig. 6 from Gibb et al., 2018
heart tube mef2cb expression increased amount, abnormal	hey2^hsc25/hsc25	standard conditions	Fig. 6 from Gibb et al., 2018
whole organism myh6 expression increased amount, abnormal	hey2^hsc25/hsc25	standard conditions	Fig. 1 from Gibb et al., 2018
anterior lateral plate mesoderm nkx2.5 expression increased amount, abnormal	hey2^hsc25/hsc25	standard conditions	Fig. 6 from Gibb et al., 2018
heart tube nkx2.5 expression increased amount, abnormal	hey2^hsc25/hsc25	standard conditions	Fig. 6 from Gibb et al., 2018
primitive heart tube myl7 expression increased distribution, abnormal	hey2^hsc25/hsc25	standard conditions	Fig. 2 from Gibb et al., 2018
anterior lateral plate mesoderm mef2cb expression increased amount, abnormal	hey2^hsc25/hsc25	standard conditions	Fig. 6 from Gibb et al., 2018
pericardium edematous, abnormal	hey2^hsc25/hsc25	standard conditions	Fig. 1 from Gibb et al., 2018
bulbus arteriosus cell population proliferation increased occurrence, abnormal	hey2^hsc25/hsc25; fb7Tg	control	Fig. 7 from Gibb et al., 2018
atrium cell population proliferation increased occurrence, abnormal	hey2^hsc25/hsc25; fb7Tg	control	Fig. 7 from Gibb et al., 2018
cardiac muscle cell cell population proliferation increased occurrence, abnormal	hey2^hsc25/hsc25; fb7Tg	control	Fig. 7 from Gibb et al., 2018
cardiac ventricle cell population proliferation increased occurrence, abnormal	hey2^hsc25/hsc25; fb7Tg	control	Fig. 7 from Gibb et al., 2018
atrium cardiac muscle cell increased amount, abnormal	hey2^hsc25/hsc25; hsc4Tg	standard conditions	Fig. 2 from Gibb et al., 2018
cardiac muscle cell increased amount, abnormal	hey2^hsc25/hsc25; hsc4Tg	standard conditions	Fig. 2 from Gibb et al., 2018
cardiac ventricle cardiac muscle cell increased amount, abnormal	hey2^hsc25/hsc25; hsc4Tg	standard conditions	Fig. 2 from Gibb et al., 2018
blood cell accumulation trunk vasculature, abnormal	hey2^hsc25/hsc25; sd2Tg	standard conditions	Fig. 1 from Gibb et al., 2018
atrium increased size, abnormal	hey2^hsc25/hsc25; twu34Tg	standard conditions	Fig. 1 from Gibb et al., 2018
heart contraction decreased rate, abnormal	hey2^hsc25/hsc25; twu34Tg	standard conditions	Fig. 1 from Gibb et al., 2018
heart looping decreased occurrence, abnormal	hey2^hsc25/hsc25; twu34Tg	standard conditions	Fig. 1 from Gibb et al., 2018
cardiac ventricle decreased size, abnormal	hey2^hsc25/hsc25; twu34Tg	standard conditions	Fig. 1 from Gibb et al., 2018

Citations