PUBLICATION
Hey2 regulates the size of the cardiac progenitor pool during vertebrate heart development
- Authors
- Gibb, N., Lazic, S., Yuan, X., Deshwar, A.R., Leslie, M., Wilson, M.D., Scott, I.C.
- ID
- ZDB-PUB-181026-14
- Date
- 2018
- Source
- Development (Cambridge, England) 145(22): (Journal)
- Registered Authors
- Deshwar, Ashish, Gibb, Natalie, Lazic, Savo, Leslie, Meaghan, Scott, Ian, Yuan, Xuefei
- Keywords
- Cardiac progenitors, Heart development, Hey2, Second heart field, Zebrafish
- MeSH Terms
-
- Animals
- Basic Helix-Loop-Helix Transcription Factors/genetics
- Basic Helix-Loop-Helix Transcription Factors/metabolism*
- Cardiovascular Diseases/pathology
- Cell Count
- Cell Lineage
- Cell Proliferation
- Cell Size
- Fibroblast Growth Factors/metabolism
- Gene Expression Regulation, Developmental
- Heart/embryology*
- Mutation/genetics
- Myocardium/metabolism
- Myocardium/pathology
- Myocytes, Cardiac/metabolism
- Myocytes, Cardiac/pathology
- Signal Transduction
- Stem Cells/cytology*
- Stem Cells/metabolism*
- Zebrafish/embryology*
- Zebrafish/genetics
- Zebrafish/metabolism*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- PubMed
- 30355727 Full text @ Development
Citation
Gibb, N., Lazic, S., Yuan, X., Deshwar, A.R., Leslie, M., Wilson, M.D., Scott, I.C. (2018) Hey2 regulates the size of the cardiac progenitor pool during vertebrate heart development. Development (Cambridge, England). 145(22):.
Abstract
A key event in heart development is the timely addition of cardiac progenitor cells, defects in which can lead to congenital heart defects. However, how the balance and proportion of progenitor proliferation versus addition to the heart is regulated remains poorly understood. Here we demonstrate that Hey2 functions to regulate the dynamics of cardiac progenitor addition to the zebrafish heart. We found that the previously noted increase in myocardial cell number found in the absence of Hey2 function was due to a pronounced expansion in the size of the cardiac progenitor pool. Expression analysis and lineage tracing of hey2-expressing cells showed that hey2 is active in cardiac progenitors. Hey2 acted to limit proliferation of cardiac progenitors, prior to heart tube formation. Use of a transplantation approach demonstrated a likely cell autonomous (in cardiac progenitors) function for Hey2. Taken together, our data suggests a previously unappreciated role for Hey2 in controlling the proliferative capacity of cardiac progenitors, affecting the subsequent contribution of late-differentiating cardiac progenitors to the developing vertebrate heart.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping