CRISPR

CRISPR1-cftr

ID

ZDB-CRISPR-181109-2

Name

CRISPR1-cftr

Previous Names

None

Target

cftr (1)

Sequence

5' - GGATGCAGAGATCACCTGTGG - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

None

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
scu101	cftr

Expression

Gene expression in Wild Types + CRISPR1-cftr

No data available

Phenotype

Phenotype resulting from CRISPR1-cftr

No data available

Phenotype of all Fish created by or utilizing CRISPR1-cftr

Phenotype	Fish	Conditions	Figures
whole organism smyhc2 expression increased amount, abnormal	cftr^{scu101/scu101}	standard conditions	Fig. 3 from Liu et al., 2020
heart contraction decreased rate of occurrence, abnormal	cftr^{scu101/scu101}	standard conditions	Fig. 1 from Liu et al., 2020
heart edematous, abnormal	cftr^{scu101/scu101}	standard conditions	Fig. 1 from Liu et al., 2020
heart dysplastic, abnormal	cftr^{scu101/scu101}	standard conditions	Fig. 1 from Liu et al., 2020
atrium dilated, abnormal	cftr^{scu101/scu101}	standard conditions	Fig. 1 from Liu et al., 2020
whole organism si:ch211-211k8.4 expression decreased amount, abnormal	cftr^{scu101/scu101}	standard conditions	Fig. 3 from Liu et al., 2020
heart looping process quality, abnormal	cftr^{scu101/scu101}	standard conditions	Fig. 1 from Liu et al., 2020
whole organism hip1ra expression decreased amount, abnormal	cftr^{scu101/scu101}	standard conditions	Fig. 3 from Liu et al., 2020
whole organism tpm4b expression increased amount, abnormal	cftr^{scu101/scu101}	standard conditions	Fig. 3 from Liu et al., 2020
whole organism itgb1b.1 expression increased amount, abnormal	cftr^{scu101/scu101}	standard conditions	Fig. 3 from Liu et al., 2020
whole organism itgbl1 expression decreased amount, abnormal	cftr^{scu101/scu101}	standard conditions	Fig. 3 from Liu et al., 2020
whole organism has2 expression decreased amount, abnormal	cftr^{scu101/scu101}	standard conditions	Fig. 1 from Liu et al., 2020
heart has2 expression decreased distribution, abnormal	cftr^{scu101/scu101}	standard conditions	Fig. 1 from Liu et al., 2020
whole organism nkx2.5 expression decreased amount, abnormal	cftr^{scu101/scu101}	standard conditions	Fig. 1 from Liu et al., 2020
whole organism cacna2d2a expression decreased amount, abnormal	cftr^{scu101/scu101}	standard conditions	Fig. 3 from Liu et al., 2020
whole organism atp2a2a expression decreased amount, abnormal	cftr^{scu101/scu101}	standard conditions	Fig. 3 from Liu et al., 2020
lateral mesoderm nkx2.5 expression decreased distribution, abnormal	cftr^{scu101/scu101}	standard conditions	Fig. 1 , Fig. 4 from Liu et al., 2020
whole organism itga10 expression decreased amount, abnormal	cftr^{scu101/scu101}	standard conditions	Fig. 3 from Liu et al., 2020
whole organism mamdc2b expression increased amount, abnormal	cftr^{scu101/scu101}	standard conditions	Fig. 3 from Liu et al., 2020
whole organism bmp4 expression decreased amount, abnormal	cftr^{scu101/scu101}	standard conditions	Fig. 1 from Liu et al., 2020
heart bmp4 expression decreased distribution, abnormal	cftr^{scu101/scu101}	standard conditions	Fig. 1 from Liu et al., 2020
primordial germ cell mislocalised, abnormal	cftr^{scu101/scu101} (AB)	standard conditions	Fig. 2 , Fig. 4 , Fig. 5 from Liao et al., 2018
whole organism rgs14a expression increased amount, abnormal	cftr^{scu101/scu101} (AB)	standard conditions	Fig. 5 from Liao et al., 2018
whole organism cxcr4b expression increased amount, abnormal	cftr^{scu101/scu101} (AB)	standard conditions	Fig. 5 from Liao et al., 2018
whole organism cftr expression decreased amount, abnormal	cftr^{scu101/scu101} (AB)	standard conditions	Fig. 1 from Liao et al., 2018
germ cell migration decreased occurrence, abnormal	cftr^{scu101/scu101} (AB)	standard conditions	Fig. 2 , Fig. 4 , Fig. 5 from Liao et al., 2018
whole organism cxcl12a expression increased amount, abnormal	cftr^{scu101/scu101} (AB)	standard conditions	Fig. 5 from Liao et al., 2018
tail bud lacks all parts of type Kupffer's vesicle, abnormal	cftr^{scu101/scu101} (AB)	standard conditions	Fig. 1 from Liao et al., 2018
Kupffer's vesicle development arrested, abnormal	cftr^{scu101/scu101} (AB)	standard conditions	Fig. 1 from Liao et al., 2018
whole organism ca15b expression increased amount, abnormal	cftr^{scu101/scu101} (AB)	standard conditions	Fig. 5 from Liao et al., 2018

Citations