CRISPR

CRISPR1-rbbp4

ID

ZDB-CRISPR-181022-1

Name

CRISPR1-rbbp4

Previous Names

None

Target

rbbp4 (1)

Sequence

5' - GATGACCCACGCGCTTGAG - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

None

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
is60	rbbp4

Expression

Gene expression in Wild Types + CRISPR1-rbbp4

No data available

Phenotype

Phenotype resulting from CRISPR1-rbbp4

Phenotype	Fish	Figures
optic tectum nucleus broken, abnormal	kca66Tg + CRISPR1-rbbp4	Fig. 6 from Schultz et al., 2018
optic tectum nucleus increased size, abnormal	kca66Tg + CRISPR1-rbbp4	Fig. 6 from Schultz et al., 2018

Phenotype of all Fish created by or utilizing CRISPR1-rbbp4

Phenotype	Fish	Conditions	Figures
head decreased size, abnormal	rbbp4^is60/is60	standard conditions	Fig. 2 from Schultz-Rogers et al., 2022
retina ab1-casp3 labeling increased amount, abnormal	rbbp4^is60/is60	control	Fig. 5 from Schultz et al., 2018
optic tectum apoptotic process increased occurrence, abnormal	rbbp4^is60/is60	control	Fig. 5 from Schultz et al., 2018
midbrain apoptotic, abnormal	rbbp4^is60/is60	standard conditions	Fig. 4, Fig. 8, Fig. 9, Fig. 11 from Schultz-Rogers et al., 2022
ciliary marginal zone cell population proliferation decreased occurrence, abnormal	rbbp4^is60/is60	standard conditions	Fig. 5 from Schultz-Rogers et al., 2022
midbrain decreased size, abnormal	rbbp4^is60/is60	standard conditions	Fig. 4 from Schultz-Rogers et al., 2022
ciliary marginal zone atoh7 expression increased distribution, abnormal	rbbp4^is60/is60	standard conditions	Fig. 6 from Schultz-Rogers et al., 2022
optic tectum apoptotic, abnormal	rbbp4^is60/is60	standard conditions	Fig. 3, Fig. 4 from Schultz-Rogers et al., 2022
retina apoptotic process increased occurrence, abnormal	rbbp4^is60/is60	control	Fig. 5 from Schultz et al., 2018
eye decreased size, abnormal	rbbp4^is60/is60	control	Fig. 2 from Schultz-Rogers et al., 2022 Fig. 5 from Schultz et al., 2018
retina cell population proliferation increased occurrence, abnormal	rbbp4^is60/is60	standard conditions	Fig. 4, Fig. 11 from Schultz-Rogers et al., 2022
ciliary marginal zone cdkn1ca expression absent, abnormal	rbbp4^is60/is60	standard conditions	Fig. 6 from Schultz-Rogers et al., 2022
ciliary marginal zone ccnd1 expression increased distribution, abnormal	rbbp4^is60/is60	standard conditions	Fig. 6 from Schultz-Rogers et al., 2022
retina decreased size, abnormal	rbbp4^is60/is60	standard conditions	Fig. 5 from Schultz-Rogers et al., 2022
midbrain cell population proliferation increased occurrence, abnormal	rbbp4^is60/is60	standard conditions	Fig. 4, Fig. 7 from Schultz-Rogers et al., 2022
brain decreased size, abnormal	rbbp4^is60/is60	control	Fig. 2 from Schultz-Rogers et al., 2022 Fig. 5 from Schultz et al., 2018
optic tectum decreased size, abnormal	rbbp4^is60/is60	standard conditions	Fig. 4 from Schultz-Rogers et al., 2022
ceratobranchial cartilage malformed, abnormal	rbbp4^is60/is60	standard conditions	Fig. 2 from Schultz-Rogers et al., 2022
optic tectum ab1-casp3 labeling increased amount, abnormal	rbbp4^is60/is60	control	Fig. 5 from Schultz et al., 2018
ceratohyal cartilage malformed, abnormal	rbbp4^is60/is60	standard conditions	Fig. 2 from Schultz-Rogers et al., 2022
retina apoptotic, abnormal	rbbp4^is60/is60	standard conditions	Fig. 4, Fig. 8, Fig. 9, Fig. 11 from Schultz-Rogers et al., 2022
ciliary marginal zone stem cell mislocalised, abnormal	rbbp4^is60/is60	standard conditions	Fig. 5 from Schultz-Rogers et al., 2022
optic tectum nucleus increased size, abnormal	kca6Tg + CRISPR1-rbbp4	control	Fig. 6 from Schultz et al., 2018
optic tectum nucleus broken, abnormal	kca6Tg + CRISPR1-rbbp4	control	Fig. 6 from Schultz et al., 2018
optic tectum nucleus increased size, abnormal	kca66Tg + CRISPR1-rbbp4	control	Fig. 6 from Schultz et al., 2018
optic tectum nucleus broken, abnormal	kca66Tg + CRISPR1-rbbp4	control	Fig. 6 from Schultz et al., 2018
midbrain apoptotic, ameliorated	rbbp4^is60/is60 + MO4-tp53	standard conditions	Fig. 9 from Schultz-Rogers et al., 2022
retina apoptotic, ameliorated	rbbp4^is60/is60 + MO4-tp53	standard conditions	Fig. 9 from Schultz-Rogers et al., 2022
retina cell population proliferation increased occurrence, exacerbated	rb1^is54/is54; rbbp4^is60/is60	standard conditions	Fig. 7 from Schultz-Rogers et al., 2022
retina apoptotic, abnormal	rb1^is54/is54; rbbp4^is60/is60	standard conditions	Fig. 8 from Schultz-Rogers et al., 2022
midbrain cell population proliferation increased occurrence, exacerbated	rb1^is54/is54; rbbp4^is60/is60	standard conditions	Fig. 7 from Schultz-Rogers et al., 2022
ceratohyal cartilage morphology, ameliorated	rbbp4^is60/is60; is61Tg	standard conditions	Fig. 2 from Schultz-Rogers et al., 2022
brain normal size, ameliorated	rbbp4^is60/is60; is61Tg	standard conditions	Fig. 2 from Schultz-Rogers et al., 2022
eye normal size, ameliorated	rbbp4^is60/is60; is61Tg	standard conditions	Fig. 2 from Schultz-Rogers et al., 2022
ceratobranchial cartilage morphology, ameliorated	rbbp4^is60/is60; is61Tg	standard conditions	Fig. 2 from Schultz-Rogers et al., 2022
head normal size, ameliorated	rbbp4^is60/is60; is61Tg	standard conditions	Fig. 2 from Schultz-Rogers et al., 2022
retina cell population proliferation normal occurrence, ameliorated	rbbp4^is60/is60; tp53^is55/is55	standard conditions	Fig. 11 from Schultz-Rogers et al., 2022
retina apoptotic, ameliorated	rbbp4^is60/is60; tp53^is55/is55	standard conditions	Fig. 11 from Schultz-Rogers et al., 2022
retina cell population proliferation increased occurrence, abnormal	rbbp4^is60/is60; tp53^is55/is55	standard conditions	Fig. 11 from Schultz-Rogers et al., 2022
midbrain apoptotic, ameliorated	rbbp4^is60/is60; tp53^is55/is55	standard conditions	Fig. 11 from Schultz-Rogers et al., 2022

Citations