PUBLICATION
Rbbp4 loss disrupts neural progenitor cell cycle regulation independent of Rb and leads to Tp53 acetylation and apoptosis
- Authors
- Schultz-Rogers, L.E., Thayer, M.L., Kambakam, S., Wierson, W.A., Helmer, J.A., Wishman, M.D., Wall, K.A., Greig, J.L., Forsman, J.L., Puchhalapalli, K., Nair, S., Weiss, T.J., Luiken, J.M., Blackburn, P.R., Ekker, S.C., Kool, M., McGrail, M.
- ID
- ZDB-PUB-220311-14
- Date
- 2022
- Source
- Developmental Dynamics : an official publication of the American Association of Anatomists 251(8): 1267-1290 (Journal)
- Registered Authors
- Blackburn, Patrick, Ekker, Stephen C., McGrail, Maura
- Keywords
- none
- MeSH Terms
-
- Acetylation
- Animals
- Apoptosis/genetics
- Cell Cycle/genetics
- Humans
- Neural Stem Cells*/metabolism
- Retinoblastoma-Binding Protein 4*/genetics
- Retinoblastoma-Binding Protein 4*/metabolism
- Transcription Factors/metabolism
- Tumor Suppressor Protein p53*/genetics
- Tumor Suppressor Protein p53*/metabolism
- Zebrafish*/genetics
- Zebrafish*/metabolism
- Zebrafish Proteins
- PubMed
- 35266256 Full text @ Dev. Dyn.
Citation
Schultz-Rogers, L.E., Thayer, M.L., Kambakam, S., Wierson, W.A., Helmer, J.A., Wishman, M.D., Wall, K.A., Greig, J.L., Forsman, J.L., Puchhalapalli, K., Nair, S., Weiss, T.J., Luiken, J.M., Blackburn, P.R., Ekker, S.C., Kool, M., McGrail, M. (2022) Rbbp4 loss disrupts neural progenitor cell cycle regulation independent of Rb and leads to Tp53 acetylation and apoptosis. Developmental Dynamics : an official publication of the American Association of Anatomists. 251(8):1267-1290.
Abstract
Background Retinoblastoma-binding protein 4 (Rbbp4) is a component of transcription regulatory complexes that control cell cycle gene expression. Previous work indicated Rbbp4 cooperates with the Rb tumor suppressor to block cell cycle entry. Here, we use genetic analysis to examine the interactions of Rbbp4, Rb and Tp53 in zebrafish neural progenitor cell cycle regulation and survival.
Results Rbbp4 is upregulated across the spectrum of human embryonal and glial brain cancers. Transgenic rescue of rbbp4 mutant embryos shows Rbbp4 is essential for zebrafish neurogenesis. Rbbp4 loss leads to apoptosis and γ-H2AX in the developing brain that is suppressed by tp53 knockdown or maternal zygotic deletion. Mutant retinal neural precursors accumulate in M phase and fail to initiate G0 gene expression. rbbp4; rb1 mutants show an additive effect on the number of M phase cells. In rbbp4 mutants Tp53 acetylation is detected, however, Rbbp4 overexpression did not rescue DNA damage induced apoptosis.
Conclusion Rbbp4 is necessary for neural progenitor cell cycle progression and initiation of G0 independent of Rb. Tp53-dependent apoptosis in the absence of Rbpb4 correlates with Tp53 acetylation. Together these results suggest Rbbp4 is required for cell cycle exit and contributes to neural progenitor survival through regulation of Tp53 acetylation. This article is protected by copyright. All rights reserved.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping