CRISPR

CRISPR3-flt1

ID

ZDB-CRISPR-170310-1

Name

CRISPR3-flt1

Previous Names

sgRNAflt1E3 (1)

Target

flt1 (1)

Sequence

5' - GGGACGGTGGGAGCTCCAGT - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

None

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
ka601	flt1
ka602	flt1
ka603	flt1
ka604	flt1
ka611Tg	flt1

Expression

Gene expression in Wild Types + CRISPR3-flt1

No data available

Phenotype

Phenotype resulting from CRISPR3-flt1

No data available

Phenotype of all Fish created by or utilizing CRISPR3-flt1

Phenotype	Fish	Conditions	Figures
intersegmental vessel dorsal region increased branchiness, abnormal	flt1^ka601/+; s843Tg	standard conditions	Fig. 2 from Wild et al., 2017
intersegmental vessel vascular sprouts increased amount, abnormal	flt1^ka601/+; s843Tg	standard conditions	Fig. 2 from Wild et al., 2017
intersegmental vein vascular sprouts increased amount, abnormal	flt1^ka601/+; s843Tg	standard conditions	Fig. 2 from Wild et al., 2017
intersegmental vessel vascular sprouts increased amount, abnormal	flt1^ka601/ka601; s843Tg	chemical treatment: LY-411575	Fig. 6 from Wild et al., 2017
intersegmental vein vascular sprouts increased amount, abnormal	flt1^ka601/ka601; s843Tg	standard conditions	Fig. 2 , Fig. 3 , Fig. 5 , Fig. 6 , Fig. 8 from Wild et al., 2017
intersegmental artery vascular sprouts increased amount, abnormal	flt1^ka601/ka601; s843Tg	chemical treatment: LY-411575	Fig. 6 from Wild et al., 2017
intersegmental vessel vascular sprouts displaced to neural tube, abnormal	flt1^ka601/ka601; s843Tg	standard conditions	Fig. 7 from Wild et al., 2017
intersegmental vessel dorsal region increased branchiness, abnormal	flt1^ka601/ka601; s843Tg	standard conditions	Fig. 2 , Fig. 5 , Fig. 6 , Fig. 8 from Wild et al., 2017
intersegmental vessel vascular sprouts increased amount, abnormal	flt1^ka601/ka601; s843Tg	standard conditions	Fig. 2 , Fig. 5 , Fig. 7 from Wild et al., 2017
intersegmental artery endothelial cell increased amount, abnormal	flt1^ka601/ka601; y7Tg	standard conditions	Fig. 3 from Wild et al., 2017
dorsal longitudinal anastomotic vessel endothelial cell increased amount, abnormal	flt1^ka601/ka601; y7Tg	standard conditions	Fig. 3 from Wild et al., 2017
dorsal aorta endothelial cell increased amount, abnormal	flt1^ka601/ka601; y7Tg	standard conditions	Fig. 3 from Wild et al., 2017
intersegmental vein endothelial cell increased amount, abnormal	flt1^ka601/ka601; y7Tg	standard conditions	Fig. 3 from Wild et al., 2017
posterior cardinal vein endothelial cell increased amount, abnormal	flt1^ka601/ka601; y7Tg	standard conditions	Fig. 3 from Wild et al., 2017
intersegmental vessel dorsal region increased branchiness, abnormal	flt1^ka602/ka602; s843Tg	standard conditions	Fig. 2 from Wild et al., 2017
intersegmental vessel vascular sprouts increased amount, abnormal	flt1^ka602/ka602; s843Tg	standard conditions	Fig. 2 from Wild et al., 2017
intersegmental vessel dorsal region increased branchiness, abnormal	flt1^ka603/ka603; s843Tg	standard conditions	Fig. 2 from Wild et al., 2017
intersegmental vessel vascular sprouts increased amount, abnormal	flt1^ka603/ka603; s843Tg	standard conditions	Fig. 2 from Wild et al., 2017
intersegmental vessel dorsal region branchiness, ameliorated	flt1^ka601/ka601; s843Tg + MO1-vegfaa	standard conditions	Fig. 8 from Wild et al., 2017
intersegmental vessel vascular sprouts amount, ameliorated	flt1^ka601/ka601; s843Tg + MO1-vegfaa	standard conditions	Fig. 8 from Wild et al., 2017
intersegmental vein vascular sprouts increased amount, exacerbated	flt1^ka601/ka601; s843Tg + MO3-dll4	standard conditions	Fig. 5 from Wild et al., 2017
intersegmental vein increased amount, abnormal	flt1^ka601/ka601; s843Tg + MO3-dll4	standard conditions	Fig. 5 from Wild et al., 2017
intersegmental vein dorsal region branchiness, exacerbated	flt1^ka601/ka601; s843Tg + MO3-dll4	standard conditions	Fig. 5 from Wild et al., 2017
intersegmental vessel dorsal region branchiness, exacerbated	flt1^ka601/ka601; s843Tg + MO3-dll4	standard conditions	Fig. 5 from Wild et al., 2017
intersegmental vessel vascular sprouts increased amount, exacerbated	flt1^ka601/ka601; s843Tg + MO3-dll4	standard conditions	Fig. 5 from Wild et al., 2017
intersegmental artery decreased amount, abnormal	flt1^ka601/ka601; s843Tg + MO3-dll4	standard conditions	Fig. 5 from Wild et al., 2017
intersegmental vessel dorsal region branchiness, ameliorated	flt1^ka601/ka601; s843Tg + MO3-flt4	standard conditions	Fig. 5 from Wild et al., 2017
intersegmental vessel vascular sprouts amount, ameliorated	flt1^ka601/ka601; s843Tg + MO3-flt4	standard conditions	Fig. 5 from Wild et al., 2017
intersegmental artery decreased diameter, abnormal	flt1^ka601/ka601; s916Tg	standard conditions	Fig. 2 from Klems et al., 2020
intersegmental artery increased diameter, abnormal	flt1^ka601/ka601; s916Tg	standard conditions	Fig. 1 from Klems et al., 2020
intersegmental artery increased diameter, abnormal	flt1^{ka611Tg/ka611Tg}; s916Tg	standard conditions	Fig. 2 from Klems et al., 2020
intersegmental vein vascular sprouts increased amount, exacerbated	vhl^{hu2117/hu2117}; flt1^ka601/ka601; s843Tg	standard conditions	Fig. 5 from Wild et al., 2017
intersegmental vessel dorsal region increased branchiness, abnormal	vhl^{hu2117/hu2117}; flt1^ka601/ka601; s843Tg	standard conditions	Fig. 7 from Wild et al., 2017
intersegmental vessel vascular sprouts increased amount, exacerbated	vhl^{hu2117/hu2117}; flt1^ka601/ka601; s843Tg	standard conditions	Fig. 5 from Wild et al., 2017
intersegmental vessel dorsal region branchiness, exacerbated	vhl^{hu2117/hu2117}; flt1^ka601/ka601; s843Tg	standard conditions	Fig. 5 from Wild et al., 2017
intersegmental artery vascular sprouts increased amount, abnormal	vhl^{hu2117/hu2117}; flt1^ka601/ka601; s843Tg	standard conditions	Fig. 5 from Wild et al., 2017
intersegmental vessel vascular sprouts displaced to neural tube, abnormal	vhl^{hu2117/hu2117}; flt1^ka601/ka601; s843Tg	standard conditions	Fig. 7 from Wild et al., 2017
intersegmental vessel vascular sprouts increased amount, abnormal	vhl^{hu2117/hu2117}; flt1^ka601/ka601; s843Tg	standard conditions	Fig. 7 from Wild et al., 2017
intersegmental artery increased diameter, exacerbated	flt1^{ka611Tg/ka611Tg}; ka613Tg; s916Tg	standard conditions	Fig. 2 from Klems et al., 2020
intersegmental vessel dorsal region branchiness, ameliorated	vhl^{hu2117/hu2117}; flt1^ka601/ka601; s843Tg + MO3-flt4	standard conditions	Fig. 5 from Wild et al., 2017
intersegmental vessel vascular sprouts amount, ameliorated	vhl^{hu2117/hu2117}; flt1^ka601/ka601; s843Tg + MO3-flt4	standard conditions	Fig. 5 from Wild et al., 2017

Citations