Fig. 2

AMBRA1 silencing in zebrafish causes aberrant cardiac looping and laterality defects. (A) Representative WMISH images for myl7 in WT and ambra1a and ambra1b ATG-morphant embryos at 20, 24, and 36 hpf developmental stages. The expected position and shape of the developing heart at the respective developmental stages are depicted in the schematic diagram on the left. Scale bar, 200 μm. (B) Dorsal views of the lateral plate mesoderm of WT and ambra1a and ambra1b ATG-morphant embryos at 19 hpf, showing different pitx2c expression patterns, as indicated by the arrowheads. Morphant embryos display disorganization of the presumptive localization of Rohon-Beard neurons (depicted in the schematic diagram on the right). Scale bar, 200 μm. n = 30 embryos for each condition. hpf, hours postfertilization; myl7, myosin light chain 7; pitx2c, homeodomain transcription factor 2 isoform c; WMISH, whole-mount in situ hybridization.