FIGURE

Figure 5

ID

ZDB-FIG-210902-145

Publication

Sarkar et al., 2021 - Involvement of Oxidative and Endoplasmic Reticulum Stress in RDH12-Related Retinopathies

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Figure 5

Retinal ultrastructure of wt and rdh12^u533 mutant fish. Transmission electron microscopy was used to assess retinal ultrastructure of wt (A,C) and rdh12^u533 (B,D) fish at 12 mpf. Phagosomes are indicated with white asterisks. Scale bar = 50 µm. (E) No significant difference was noted in the number of phagosomes between wt and rdh12^u533 fish. (F) Phagosomes were significantly larger in the rdh12^u533 fish at 12 mpf. Data are displayed as mean ± SEM. Phagosome number and size was quantified using ImageJ from three wt and rdh12^u533 fish. Statistical significance was analysed by Mann–Whitney, *** p ≤ 0.001. (G) mRNA expression of autophagy genes in the retina of 12 mpf fish was analysed by RT-qPCR. Expression of atg12 was significantly reduced in the rdh12^u533 fish. * p ≤ 0.05. OS, outer segment; RPE, retinal pigment epithelium; BM, Bruch’s membrane.

Expression Data

Genes:	atg5 atg7 atg12 sqstm1
Fish:	AB rdh12^{zf3693/zf3693}
Anatomical Term:	retina
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	rdh12^{zf3693/zf3693}
Observed In:	retina retinal pigmented epithelium phagocytic vesicle
Stage:	Adult

Phenotype Detail

Acknowledgments

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