FIGURE

Fig. 3

ID

ZDB-FIG-080605-35

Publication

Kurtzman et al., 2001 - Ubc9 interacts with a nuclear localization signal and mediates nuclear localization of the paired-like homeobox protein Vsx-1 independent of SUMO-1 modification

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Fig. 3

Zebrafish Vsx-1 is not a substrate for SUMO-1 conjugation in vitro or in COS-7 cells. Open and closed arrows indicate unconjugated MT-zVsx-1 and RanGAP1, respectively. Asterisk shows RanGAP1 conjugated to endogenous SUMO-1. (A) Xenopus RanGAP1 (lanes 1-4) or MT-zVsx-1 (lanes 6-9) were in vitro translated and labeled with [³⁵S]Met/[³⁵S]Cys. Recombinant GST-SUMO-1 and Ubc9 were added as indicated. Ubc9 (3x) was added to lanes 4, 5, 9, and 10. Western blots using anti-SUMO-1 or anti-hsUbc9 antibodies show relative levels of GST-SUMO-1 and Ubc9. (B) COS-7 cells were transfected with expression plasmids encoding RanGAP1, MT-zVsx-1, and GFP-SUMO-1. Cell lysates were immunoblotted with anti-RanGAP1 antibody (lanes 1 and 2 in upper gel), 9e10 antibody (lanes 3-5 in upper gel), or anti-GFP antibody (lower gel). Identical results were observed when GFP-SUMO-2 was substituted for GFP-SUMO-1.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA