FIGURE

Fig. 2

ID

ZDB-FIG-080605-34

Publication

Kurtzman et al., 2001 - Ubc9 interacts with a nuclear localization signal and mediates nuclear localization of the paired-like homeobox protein Vsx-1 independent of SUMO-1 modification

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Fig. 2

zUbc9 interacts with a NLS at the NH₂ terminus of the homeodomain. (A) Vsx-1 deletion mutants: 1, zVsx-1(ORF); 2, zVsx-1ΔC-term; 3, goldfish Vsx-1 target; 4, zVsx-1ΔCVC; 5, zVsx-1ΔN-term1; 6, zVsx-1ΔN-term2; 7, zVsx-1ΔNLS/HD; and 8, zVsx-1ΔNLS. (B) Liquid culture assay using CPRG as substrate (CLONTECH) was used to calculate β-galactosidase activity. One unit of β-galactosidase equals the amount that hydrolyzes 1 μmol of CPRG to chlorophenol red and D-galactose per minute per cell. In the graph, the means (±SEM) are the result of samples processed in triplicate. (C) Anti-LexA antibody Western blot of yeast lysates shows expression of the LexA- binding domain fusion proteins noted in A.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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