For the most current and complete antibody data, use the Antibody Search Page
To see ZIRC antibodies only, set the search to "Show only ZIRC antibodies". Monoclonal antibodies that recognize a variety of structures in zebrafish embryos are available from the library at the Zebrafish International Resource Center. Please feel free to communicate with us via email if you have any questions: fish_requests@zebrafish.org
To see ZIRC antibodies only, set the search to "Show only ZIRC antibodies". Monoclonal antibodies that recognize a variety of structures in zebrafish embryos are available from the library at the Zebrafish International Resource Center. Please feel free to communicate with us via email if you have any questions: fish_requests@zebrafish.org
The following is a partial list of available antibodies at ZIRC:
(Source: K. Larison and B. Trevarrow)
(Abbreviations are listed at the end.)
Name | Type | Staining | Structures stained | |
anti-Dlx3b | IgG1,k | nuclear | neural plate border, otic preplacode, otic placode, olfactory, finfold | |
anti-Vegt (aka "anti-Spadetail") |
IgG2a,k | nuclear | tailbud & adaxial cells; presomitic, lateral mesoderm, and prechordal plate cells | |
zl-1 | IgG1 | cyto or ecm | lns | |
zm-1 | gran cyto | mus,act?,sheet mus over yolk(96h),fnv,hrt,musoc,muse,musb,fnpec,fnv | ||
zm-2 | gran cyto | mus/sub skin of fnd,act,sheet over cns(96h) | ||
zm-3 | gran cyto | som,mus,myo,str,musb,muse,musoc | ||
zn-1 | IgG1 | cyto | blm,ysl,ne u,axn,har,drg,rbn,pmn,ens,pns | |
zn-2 | surf | npl, lns,hin clusters,spi roots,har,for cells, tri, trg, npl, rbn, visc organ | ||
zn-3 | surf | proc,npl, neu,cns surf,pns,hin segs,fnp,ens,pns,npl,bns,ipl,opl,some nerve tracts | ||
zn-4 | cyto or surf | neu,har,npl,proc,pin,hin npl seg,spi seg?,fin cells & proc, orc,lining of visc organ, outside of ins, ncr(?), ipl, gan, eye neu | ||
zn-5 | IgG1 | surf | rbn, hin clusters, vsn, mps, per gang cell, gan, some cns pathways, ncr(?), npl | |
zn-8 | IgG1 | surf | periodic cells between som surf & skin, hsp, some cells in brn, rbn, vsn, som, hin clusters | |
zn-9 | IgG1 | surf(?) | hin verticle proc seg,tri,trg,sln,hin npl,ens, pns, opl, fin(lt) | |
zn-12 | IgG1 | surf | rbn,dsn,proc in cns & pns, mus, myc, nrt, hin clusters, fin, bns, ens, ntd(1 month) | |
zn-13 | IgM | surf | rbn,dsn,trg,tri,mus,myc,har,npl(lt),ens | |
zn-15 | IgG1 | cyto or surf(?) | proc, npl, neu,hin seg, har, pin, cns, pns, ens, pln, opl, ipl, blood vessels in brn(?) | |
zna-1 | IgG1 | fibers,npl,hin seg,growth cones, mus spag, some somata | ||
znp-1 | IgG2a,k | surf | npl,vent roots,hin seg,ipl,mus spag,ysl, some spi cells | |
znp-2 | IgG1 | ipl,opl,base of har,spi npl,bnp | ||
znp-3 | npl,bnp,snv | |||
znp-6 | evl,ysl,cns,nrt,lns,som,npl,sln,svn,myc,visc organs, gills (nerves?) | |||
znp-7 | surf | npl,bnp,snl,snv,ipl,opl(double layer),hin seg, ysl, har(base), some neu in nrt & spi | ||
zns-1 | IgG1 | cyto(?) surf | cns,pns,cells & proc,hin seg,ens,opl,ipl,growing edge of retina stains different from rest | |
zns-2 | surf | npl,bnp,raf in hin,har,olf,opl,ipl,ens,act or fin proc | ||
zns-3 | IgG1 | cyto,surf(?) | ysl,mus,per,neu,har,pns,str | |
zns-5 | surf | cns,oto,har,pns,ens,bns,fin fibers,opl,ipl,hin seg | ||
zns-6 | cns,fibers,raf,npl,opl,ipl,myc,ect,hin seg | |||
zns-7 | cyto | evl,ysl,dee,lns,cns,ntd,som,gut,npl ,cells in oral epithelium,hsp,myc | ||
znt-1 | IgM | cyto or ecm | ntd,around lns,act fnd & fnv,myc,btw ect & som,fnp tip,npl,har,part of oto,ret edge,ipl | |
znu-1 | nuclei,chromosomes,some cyto, all tissues except possibly ysl | |||
znu-2 | nuclei,dee,evl,outer layer of lns,rbn,nrt, scattered cells in brn | |||
zpr-1 | cyto(?) | phr, pin, long double cones | ||
zpr-2 | cyto | pin,phr | ||
zpr-3 | cyto(?) | phr,rod outer segments,many other tissues at 1 month | ||
zrf-1 | IgG1 | cyto(?) | rafv spi & hin seg, vent roots, dor-vent tract in brn, ntd(lt), lining around gut, raf in nrt | |
zrf-2? | cyto(?) | raf thru cns dker in spi & hin | ||
zrf-3 | IgG1 | surf | raf,brn edge,hin seg raf,pln,ipl,opl,sln, pattern on top of brn | |
zrf-4 | surf | raf,hin seg raf,some cell outlines,cns,optic nerve | ||
zs-2 | cyto(?) | outer lns,nrt,fiber tract in brn,inner segs of rod(kdl), nrt npl(lt) | ||
zs-3 | cyto | nrt | ||
zs-4 | cyto | evl,ysl,dee,cns,lns,mus,fiber tract in brn,outer layer of lns cells,gan & other nrt cells,iplk,opl, adult nrt only rod inner segm ents(cryo-kdl) |
ABBREVIATIONS | ||
act | actinotrichia | |
alg | anterior lateral line ganglion | |
aln | anterior lateral line nerve | |
alp | anterior lateral line primordium | |
ama | retinal amacrine neuron | |
axn | axon | |
bip | retinal bipolar neuron | |
bld | blood | |
blm | blastomere | |
bnp | brain neuropil | |
bns | branchial nervous system | |
bra | branchial arch | |
brn | brain | |
brp | branchial pouch | |
cad | caudal hindbrain level | |
cap | caudal primary motoneuron | |
cns | central nervous system | |
den | dendrite | |
drg | dorsal root ganglia | |
dsn | dorsal spinal cord neuron | |
ect | ectoderm | |
end | endoderm | |
ens | enteric nervous system | |
ent | endothelium | |
epb | epiblast | |
epi | epidermis | |
epn | ependymal cells | |
eye | eye | |
fin | fin | |
fbr | forebrain | |
fnc | caudal fin | |
fnd | dorsal fin | |
fnl | pelvic fin | |
fnp | pectoral fin | |
fnv | ventral fin | |
gan | retinal ganglion neuron | |
ger | germ ring | |
gli | glia | |
har | hair cell | |
hat | hatching gland | |
hbr | hindbrain | |
hor | retinal horizontal cell | |
hrt | heart | |
hyb | hypoblast | |
int | interneuron | |
ipl | inner plexiform layer | |
kee | keel | |
lns | lens | |
mar | blastoderm margin | |
max | Mauthner axon | |
mbr | midbrain | |
mec | mesenchyme | |
mel | melanocyte | |
mes | mesoderm | |
mi1 | middle hindbrain level 1 | |
mi2 | middle hindbrain level 2 | |
mi3 | middle hindbrain level 3 | |
mid | mid neuron | |
mip | middle primary motoneuron | |
miv | miv neuron | |
mld | Mauthner lateral dendrite | |
mot | motoneuron | |
mps | muscle pioneers | |
mth | Mauthner neuron | |
mul | Muller cell in retina | |
mus | muscle | |
musb | branchial muscle | |
muse | enteric muscle | |
musep | epaxial muscle | |
mush | hypaxial muscle | |
musoc | extra-ocular muscle | |
mussf | muscle surface | |
mvd | Mauthner ventral dendrite | |
myc | myocomma cell | |
myo | myotome | |
ncr | neural crest cell | |
ner | nervous tissue | |
neu | neuron | |
nms | neuromast | |
npl | neuropil | |
nrt | neural retina | |
ntd | notochord | |
nts | notochord sheath | |
olf | olfactory pit/primordium | |
oln | nerve innervating olfactory bulb | |
opl | outer plexiform layer | |
otc | otocyst | |
oto | otic capsule | |
per | peridermis | |
phr | photoreceptor | |
pig | pigmented cell | |
pin | pineal gland | |
plg | posterior lateral line ganglion | |
pln | posterior lateral line nerve | |
plp | posterior lateral line primordium | |
pmn | primary motoneuron | |
pne | pronephritic kidney | |
pns | peripheral nervous system | |
prd | pronephritic duct | |
prt | pigmented retina | |
raf | radial fibers | |
rbn | Rohon-Beard neuron | |
ro1 | rostral hindbrain level 1 | |
ro2 | rostral hindbrain level 2 | |
ro3 | rostral hindbrain level 3 | |
rop | rostral primary motoneuron | |
rsn | reticulospinal neuron | |
shd | shield | |
smn | secondary motoneuron | |
snl | spinal cord lateral neuron | |
snp | spinal cord neuropil | |
snv | spinal cord ventral neuron | |
som | somite | |
spi | spinal cord | |
str | muscle striations | |
tin | t-interneuron | |
trg | trigeminal ganglion | |
tri | trigeminal nerve | |
vep | ventral ependymal plate cells | |
vsn | ventral spinal cord neuron | |
yol | yolk cell | |
ysl | yolk syncytial layer |