FIGURE SUMMARY
Title

Traditional African remedies induce hemolysis in a glucose-6-phopshate dehydrogenase deficient zebrafish model

Authors
Arogbokun, O., Shevik, M., Slusher, T., Farouk, Z., Elfstrum, A., Weber, J., Cusick, S.E., Lund, T.
Source
Full text @ Sci. Rep.
Figure 1

Compound tested in the G6PD-deficient zebrafish model. (A) Eucalyptus oil. (B) A variety of roots, bark, herbs to brew Yoruba tea (right panel). (C) Methylated spirits.
Figure 2

Hemolysis in a G6PD-deficient zebrafish model. Top micrograph shows a zebrafish at 72 hpf with intact red blood cells. Bottom micrograph shows an edematous G6PD-deficient zebrafish at 72 hpf after 48 h of methylated spirit exposure. Both animals were subject to o-dianisidine staining to show hemoglobin containing red cells. Zebrafish were imaged using a Leica DFC340FX fluorescent microscope with PlanAPO 1.6×/0.05 NA objective. Image capture was performed with Leica Application Suite X 3.6.0.2010 (https://www.leica-microsystems.com/products/microscope-software/p/leica-application-suite/).
Figure 3

Enumeration of the hemolytic phenotype in G6PD-deficient zebrafish after compound exposure. (A) Impact of eucalyptus oil exposure and regular fishwater on zebrafish in the following groups: G6PD MO, and random MO, uninjected control group. (B) Impact of methylated spirits exposure versus regular fishwater on zebrafish in the following groups: G6PD MO, and random MO, uninjected control group. (C) Impact of Yoruba tea exposure versus regular fishwater on zebrafish in the following groups: G6PD MO, and random MO, uninjected control group.
Figure 4

Treatment effect of compounds on G6PD-deficient zebrafish. Tallies were normalized to phenotype data produced in the unexposed embryos to produce a treatment effect. Random MO and G6PD MO injected animals were compared using multivariate analysis was used to separate effects from morpholino exposure versus compound exposure.
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Sci. Rep.
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