ankef1a and ankef1b are expressed in early embryos. Rows: A-C, G, H, and J are lateral views; Row D and E are animal pole view; Row F are vegetal pole views; Row I are ventral view; Row K are dorsal view. The white arrowheads at shield (D) and black arrowheads at 60% epiboly (E) indicate ankef1a and ankef1b expression along the site of involution and convergent extension during gastrulation. Note the DFCs indicated at the YPC stage (asterisk in F), which was not seen in ankef1b YPC-stage embryos. Black arrowheads in 24 hpf lateral view (J) indicates ankef1a expression along the dorsal side of the yolk extension. Otic vesicle expression of ankef1a is indicated by white arrowheads in the 24 hpf dorsal view (K). Scale bars for each stage = 200 μm.

ankef1a and ankef1b expression in 48, 96, and 120 hpf embryos. A,C,E are lateral views, while B,D, and F are dorsal views. Black arrowheads in G-H and J-K indicate pharyngeal staining. Staining in the swim bladder region in 96 and 120 hpf is denoted by white arrowheads in G-H and J-K. Arrows in G-H point to otic vesicles. Scale bars for each whole mount images A-L = 200 μm. Relative whole-mount positions corresponding to cryosections M-N are indicated by dashed line and adjacent boxed red letters in G-H and J-K. M-N demonstrate ankef1a and ankef1b expression in the otic vesicles of a 96 hpf embryos. O–P show ankef1a and ankef1b expression in the pharynx of 120 hpf embryos. Q-R indicate ankef1a and ankef1b expression in the swim bladder of 120 hpf embryos. g: gut; o.v.: otic vesicle; pa: pancreas; ph: pharynx; s.b.: swim bladder; y: yolk. Scale bars for cryosections M-R = 100 μm.

ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions.