Fig. 4

Temporal and spatial expression of zebrafish unc119a1. A. RT-PCR of unc119a at various embryonic time-points (0.75 hpf (2-cell), 3.5 hpf, 10 hpf, 13 hpf, 18 hpf, 24 hpf, 48 hpf, and 96 hpf) compared to Ef1a. B-E. 13 hpf WISH embryos. F. 18 hpf WISH. Boxed regions are represented in flatmounts of the head (F′) and tail (F″). G. 24 hpf WISH. Boxed regions are represented in flatmounts of the head (G′) and tail (G″). H. Lateral view of 96 hpf WISH. Boxed region is represented in eye flatmount (H′). I. Dorsal view of 96 hpf WISH. Abbreviations: cmz, ciliary marginal zone; icm, intermediate cell mass; inl, inner nuclear layer; le, lens; NTC, non-template control; op, olfactory pits; ov, otic vesicle; pbi, posterior blood island; pg, pineal gland; ph, photoreceptors; pm, posterior mesenchyme.
Fig. 5

Temporal and spatial expression of zebrafish unc119a2. A. RT-PCR of unc119bat various embryonic time-points (0.75 hpf (2-cell), 3.5 hpf, 10 hpf, 13 hpf, 18 hpf, 24 hpf, 48 hpf, and 96 hpf) compared to Ef1a. B. 2-cell WISH. C-D. 10 hpf WISH. E-H. 13 hpf WISH. I. 18 hpf WISH. Boxed regions are represented in flatmounts of the head (I′) and tail (I″). J. 24 hpf WISH. Boxed regions are represented in flatmounts of the head (J′) and tail (J″). K. Lateral view of 96 hpf WISH. Boxed region is represented in eye flatmount (K′). L. Dorsal view of 96 hpf WISH embryo. Abbreviations: ac, adaxial cells; cmz, ciliary marginal zone; di: diencephalon; k, kidney; le, lens; np, neural plate; NTC, non-template control; ov, otic vesicle; pd, pronephric duct; pg, pineal gland; ph, photoreceptors; sc, spinal cord; te: telencephalon.
Fig. 6

 Temporal and spatial expression of zebrafish unc119b. A. RT-PCR of unc119c at various embryonic time-points (0.75 hpf (2-cell), 3.5 hpf, 10 hpf, 13 hpf, 18 hpf, 24 hpf, 48 hpf, and 96 hpf) compared to Ef1a. B. 2-cell WISH. C-D. 10 hpf WISH. E-H. 13 hpf WISH. I. 18 hpf WISH. Boxed regions are represented in flatmounts of the head (I′) and tail (I″). J. 24 hpf WISH. Boxed regions are represented in flatmounts of the head (J′) and tail (J″). K. Lateral view of 96 hpf WISH. Boxed region is represented in eye flatmount (K′). L. Dorsal view of 96 hpf WISH. Abbreviations: cmz, ciliary marginal zone; nm, neuromasts; np, neural plate; NTC, non-template control; op, olfactory pits; ov, otic vesicle; pd, pronephric duct; pg, pineal gland; ph, photoreceptors; pm, paraxial mesoderm; sc, spinal cord.
Fig. 7

Temporal and spatial expression of zebrafish unc119c. A. RT-PCR of unc119c at various embryonic time-points (0.75 hpf (2-cell), 3.5 hpf, 10 hpf, 13 hpf, 18 hpf, 24 hpf, 48 hpf, and 96 hpf) compared to Ef1a. B. 18 somites WISH. Boxed region represented in flatmount of the head (B′). C. 24 hpf WISH. Boxed regions are represented in flatmounts of the head (C′). D. Lateral view of 96 hpf WISH. Boxed region is represented in eye flatmount (D′). E. Dorsal view of 96 hpf WISH. Abbreviations: NTC, non-template control; op, olfactory pits; ov, otic vesicle; pg, pineal gland; ph, photoreceptors.


EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Terms:
Stage Range: 2-cell to Day 4
Fig. S1

Sense probes for in situ hybridization of the zebrafish unc119 genes at select time points. Sense probes against unc119a1 (A-D), unc119a2 (E-H), unc119b (I-L), and unc119c (M-P) show no staining. Sense probes were allowed to stain for the same length of time as the antisense probes. Abbreviations: hpf, hours post-fertilization.
Fig. S2

In situ hybridization of each of the unc119 paralogues at 48 hpf. Expression of unc119a1 (A), unc119a2 (B), unc119b (C), and unc119c (D) is shown. This time point does not show expression in any tissues different from that of 24 hpf or 96 hpf. Abbreviations: hpf, hours post-fertilization; pg, pineal gland; ph, photoreceptors.


EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Long-pec
Acknowledgments
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Reprinted from Gene expression patterns : GEP, 33, Jean, F., Pilgrim, D., Evolution and expression of the zebrafish unc119 paralogues indicates a conserved role in cilia, 1-10, Copyright (2019) with permission from Elsevier. Full text @ Gene Expr. Patterns
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