Zebrafish st8sia6-like gene spatio-temporal expression during embryonic and larval development. (A) Absolute quantification of the mRNA levels of the zebrafish st8sia6-like gene was achieved by Q-PCR in ovary, and at 0, 6, 14, 24 and 36 hpf. PCR for two biological samples was carried out in triplicate as described in the materials and methods section, and data are expressed as means +/-SD. (B) The zebrafish st8sia6-like gene distribution was studied by whole mount in situ hybridization with a digoxigenin-labeled Dre st8sia6-like antisense riboprobe. Developmental stage is given as hpf and anterior is to the left: (a) gastrula stage (5 hpf); (b) early somitogenesis (10 hpf); (c) mid-somitogenesis (15 hpf), (d,e) lateral and dorsal views at pharyngula stage (24 hpf), (f,g), lateral and dorsal views at 36 hpf, (h,i) hatching time (48 hpf), (j,k), larva stage (120 hpf).