Neutrophil-Candida interactions in zebrafish. (a) Zebrafish larvae were infected with Candida by hindbrain injection and neutrophil recruitment to the hindbrain was assessed after 4 h. Experiments were performed on 3 occasions with at least 10 larvae per condition, and data were analyzed by ANOVA with pairwise comparisons using the Holm-Sidak method (*, P < 0.05; ns, not significant). (b) Transgenic larvae with neutrophils expressing cytosolic EGFP (green) and mCherry-tagged histone 2B (red) were infected with Candida by hindbrain injection and imaged after 24 h. Arrowheads indicate yeast cells. Arrows indicate intact neutrophils. Single dots show condensed nuclear material consistent with dead neutrophil fragments. The dotted line outlines the cloud of tagged histone signal. The measurement bars represent 5 µm.