FIGURE SUMMARY
Title

Zebrafish Lacking Circadian Gene per2 Exhibit Visual Function Deficiency.

Authors
Huang, D.F., Wang, M.Y., Yin, W., Ma, Y.Q., Wang, H., Xue, T., Ren, D.L., Hu, B.
Source
Full text @ Front. Behav. Neurosci.

ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions.

ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions.

PHENOTYPE:
Fish:
Observed In:
Stage: Day 6

per2 mutant zebrafish larvae exhibited normal retinal morphology and abnormal photoreceptor ribbon synapses. (A,B) AB-WT and per2−/− zebrafish larval retinas revealed relatively normal retinal morphology by IHC. Images are LSCM presentations. Larvae at 5 dpf were stained with zpr1 (green) and propidium iodide (PI; red), which label double cones and nuclei (n = 6 animals per genotype). Scale bar = 40 μm. (C–F) per2 mutant zebrafish larvae exhibited abnormal ribbon synapses. (C) In the AB-WT retina, synaptic ribbons (arrows) are associated with the presynaptic membrane via an arciform density (asterisks). (D) In the per2−/− retina, synaptic ribbons in most of the pedicles appear to be floating in the cytoplasm and are unassociated with an arciform density and the presynaptic membrane. AB-WT (E) had relatively more normal ribbon synapses (asterisks) than those of the per2 mutant (F; n = 3 retina per genotype). Scale bar = 1 μm. (G,H) Expression of cacnf1a and syjn1 was reduced in mutant compared with WT retinas (5 dpf, n = 10 animals per genotype, *P < 0.05 and ***P < 0.001, unpaired two-tailed t-tests). Data represent the mean ± SEM.

EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Anatomical Term:
Stage: Day 5
PHENOTYPE:
Fish:
Observed In:
Stage: Day 5

ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions.

EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Term:
Stage: Day 5
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Day 5

ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions.

EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Term:
Stage: Adult
PHENOTYPE:
Fish:
Observed In:
Stage: Adult
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front. Behav. Neurosci.