FIGURE SUMMARY
Title

microRNA-183 is Essential for Hair Cell Regeneration after Neomycin Injury in Zebrafish

Authors
Kim, C.W., Han, J.H., Wu, L., Choi, J.Y.
Source
Full text @ Yonsei Med. J.
Fig. 3

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Fig. 4

In situ hybridization of miR-183 after MO injection. (A) The larvae injected with standard MO shows clear miR-183 expression in neuromast cells at 122 hours post fertilization (arrows). (B) In contrast, miR-183 expression disappeared in larvae injected with MO-183. Scale bar, 50 µm. miR, miRNA; MO, morpholino.

EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagent:
Anatomical Term:
Stage: Day 5
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Day 5
Fig. 5

The effect of miR-183 knockdown on hair cell regeneration after neomycin-induced ototoxicity. (A) Hair cells labeled with Y-PRO1 are regenerating 24 h after neomycin treatment in zebrafish larvae injected with control MO. (B) Hair cells are partially regenerating in larvae injected with MO-183 (1 mM). (C) Summarized data from six independent experiments. Scale bar, 100 µm. *p<0.05. miR, miRNA; MO, morpholino.

Fig. 6

The effect of miRNA-183 knockdown on hair cell regeneration in the inner ear of pou4f3::GFP zebrafish larvae. (A) Hair cells and otoliths are mostly regenerated in larvae injected with control MO. (B) Hair cells and otoliths are partially regenerated in larvae injected with MO-183. MO, morpholino; GFP, green fluorescent protein.

Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Yonsei Med. J.
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