FIGURE SUMMARY
Title

Functional Characterization of Zebrafish (Danio rerio) Bcl10

Authors
Mazzone, P., Scudiero, I., Ferravante, A., Paolucci, M., D'Andrea, L.E., Varricchio, E., Telesio, G., De Maio, C., Pizzulo, M., Zotti, T., Reale, C., Vito, P., Stilo, R.
Source
Full text @ PLoS One
Fig. 2

A) Immunoblot analysis of lysates from HEK293 cells transfected with and expression vector empty (vector) or expressing HA-zBcl10 and HA-hBCL10. 24 hrs after transfection, cell lysates were prepared, separated by SDS/PAGE, and analyzed by immunoblot assay probed with anti-HA (left panel) and anti-BCL10 (right panel) antisera. An arrow indicates hBCL10 endogenously expressed by HEK293 cells. B) Cell lysates from HEK293 transfected cells were prepared as in A). Were indicated, before SDS/PAGE separation cell lysates were treated with 10 units of calf intestinal phosphatase (CIP) for 30 min at 37°C. C) The same experiment shown in B) was carried out in the embryonic zebrafish fibroblast cell line PAC2. D) Immunoblot analysis of proteic extracts from zebrafish organs probed with an anti-hBCL10 antisera. Cell extract from HEK293 cells transfected with zBcl10 was used as a positive control (arrow).

EXPRESSION / LABELING:
Gene:
Antibody:
Fish:
Anatomical Terms:
Stage: Adult
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One
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