Induction of liver tumors in the three zebrafish transgenic liver cancer models. (A–D) Gross morphology of treated transgenic fish and the non-transgenic siblings. The treated non-transgenic siblings have normal liver size and gross morphology (A). The livers in the treated transgenic fish were obviously enlarged (B–D) compared to the treated non-transgenic siblings (A). (E–H) Histological examination of the treated transgenic fish and non-transgenic siblings. Treated xmrk fish developed HCC (F), treated Myc fish developed HCA (G), and treated kras fish developed heterogeneous HCC (H). The liver was circled out in white dotted lines. Scale bars: 25 mm for A–D, 50 μm for E–H.