Inhibition of Dnmts suggests that the regulation of DNA methylation is important for MG reprogramming. (A) Four-day postinjury 1016 tuba1a:gfp transgenic fish treated with 5-dAza show increased levels of endogenous (unstained) GFP. (B) 5-dAza inhibits the generation and localization of PCNA-positive MG-derived progenitors. *, mark the injury site in A and B. (C) Quantification of PCNA-positive nuclei per injury site. The value is normalized to the value of the 2-dpi control. *P < 0.02873. (D) Graphic depicting the localization of PCNA-positive nuclei within the retina at the indicated time points. 5-dAza perturbs the localization of PCNA-positive nuclei at 4 and 7 dpi. In C and D error bar extending from the box represent standard deviation. *P < 0.04128. GCL, ganglion cell layer; INL, inner nuclear layer; IPL, inner plexiform layer; ONL, outer nuclear layer; OPL, outer plexiform layer. (Scale bars, 50 µm.)