Genes expressed in the adenohypophysis. (A) scg3, (B) slc16a9a, (C) hsd3b7, (D) adm2, (E) uo:ion002 and (F) uo:ion003 are expressed (blue) in the adenohypophysis that is delimited by pomc expression (red). scg3 (A) and slc16a9a (B) are expressed in the rostral pars distalis (RPD). (C) hsd3b7 is expressed in the entire gland. (D) The EST adm2 is expressed in the proximal pars distalis (PPD) and pars intermedia (PI). (E) The novel gene uo:ion002 is expressed in the PI. (F) The novel gene uo:ion003 is expressed in the rostral pars distalis (RPD). Ventral views of adenohypophysis at 72 h, anterior to the top; scale bar 50 μm.

Genes expressed in the hypothalamus. (A–D) adcyap1b and kctd4 (E–H) are expressed in the hypothalamus. adcyap1b is expressed in the telencephalon from 24 to 72 h (A–C) and in the hypothalamus at 48 and 72 h (B–C). (D) adcyap1b and kctd4 (H) are expressed by hypothalamic cells (black) close to the adenohypophysis (demarcated by pomc, red) but not in neurohypophysial cells. (I–K) camk1d is expressed in the dorsal hypothalamus, telencephalon, and midbrain at 48 and 72 h. (L–N) stmn1b is expressed in telencephalic, mesencephalic, and hypothalamic cells between 24 and 72 h. (O) The section at 72 h suggests that ventral hypothalamic cells and some, but not all neurohypophysial cells express the gene. (P–R) psd3l is also expressed in telencephalon and hypothalamus between 24 and 72 h. (S) Hypothalamic cells express psd3l. (T–V) serpini1 is expressed in the dorsal hypothalamus at 24 h (T), at 48 h in telencephalon, diencephalon, and mesencephalon. (W) serpini1 is expressed by hypothalamic cells (blue) close to the adenohypophysis (demarcated by pomc, red) but not in neurohypophysial cells. Side views, anterior to the left. (A, E, L, P, T) 24 h; (B, F, I, M, Q, U) 48 h; (C, G, J, N, R, V) 72 h; Scale bar 100 μm. (D, H, K, O, S, W) 15 μm sagittal sections through the pituitary and adjacent hypothalamus at 72 h, scale bar 50 μm.

Genes expressed in hypothalamus, adenohypophysis, and in neurohypophysis. (A–D) uo:ion003 is expressed in telencephalon, diencephalon, and mesencephalon between 24 and 72 h. (D) The section shows that hypothalamic cells and cells in the posterior pars distalis (APD and PI are demarcated by pomc, red) express uo:ion003. (E–H) crabp1a is expressed in the presumptive neurohypophysis. crabp1a is expressed close to the adenohypophysis from 24 to 72 h (E–H) and in the telencephalon and tectum at the later stages. Sagittal sections through the pituitary (pomc, red) show crabp1a expression dorsal to the adenohypophysis in neurohypophysial cells (L). Side views, anterior to the left. (A and E) 24 h; (B and F) 48 h; (C and G) 72 h; scale bar is 100 μm. (D and H) 15 μm sagittal sections, anterior to the left, dorsal up, through the pituitary and adjacent hypothalamus at 72 h; scale bar: 50 μm.

Genes expressed in adult hypothalamic–pituitary axis. (A–H) Genes isolated in the hypothalamic library expressed in the adult. (A–D) kctd4 and psd3l were expressed in adult telencephalon and hypothalamus corresponding to the expression domains we found in 1–5 day old larvae (Fig. 2E–H and P–S). (E and F) In contrast, nppcl was not expressed in the hypothalamus between 1 d and 5 d; however, we found expression in a distinct set of cells in the adult hypothalamus. (G and H) The novel gene uo:ion006 was not expressed in embryonic and larval tissues at all, however, we detected hypothalamic expression in the adult. (I–P) We made similar observations for the pituitary library: (I–L) genes such as uo:ion002 and uo:ion003 that were expressed in the embryonic larval HP were also found in the adult pituitary (I and J) or hypothalamus and pituitary (K and L). (M–P) pncr2 that was not detected in embryonic and larval HP, or nms that was not detected in embryos and larvae at all, were both detected in adult HP (M and N) and adult pituitary (O and P). (A–P) 15 μm, sagittal sections of the adult brain, anterior left, dorsal up. (A, C, E, G, I, K, M, O) scale bar 1 mm; (B, D, F, H, J, L, N, P) scale bar 500 μm.