In situ localization of α and β CaMK-II mRNAs. CaMK-II expression was assessed by in situ hybridization with a probe for camk2a1 and camk2b1 at the indicated stages. Stages analyzed in Figures 4-6 include the 8-cell stage (8; 1hpf), the 512-cell stage (512; 2.5hpf), the dome stage (Do; 4hpf), the shield stage (Sh; 6hpf), the 3-somite stage (3s; ~ 11hpf), and the 18-somite stage (18s; ~ 18hpf). The 8-cell stage is an animal poll view; others are lateral except for dorsal views at 24, 48, and 72hpf. Arrow in camk2a1 indicates heart. Arrows in camk2b1 at 24hpf indicate somites and at 72hpf gut. Letters locate cross-sections for Figure 7. Scale bar = 1 mm.

In situ localization of γ CaMK-II mRNAs. CaMK-II expression was assessed by in situ hybridization with a probe for camk2g1 and camk2g2 at the indicated stages as in Figure 4. Arrows in camk2g1 at 24hpf indicate dorsal cell bodies and at 72hpf gut. Letters locate cross-sections for Figure 7. Scale bar = 1 mm.

In situ localization of δ CaMK-II mRNAs. CaMK-II expression was assessed by in situ hybridization with a probe for camk2d1 and camk2d2 at the indicated stages as in Figure 4. The 8-cell stage is an animal poll view; others are lateral except for dorsal views at 24, 48, and 72hpf. Arrows in camk2d1 at 24hpf indicates hatching gland. Letters locate cross-sections for Figure 7. Scale bar = 1 mm.

Cross-sectional localization of CaMK-II mRNAs. CaMK-II expression was localized at 72hpf by 30-μm frozen thin sections of pre-stained embryos. Sections shown were acquired at the locations (A-F) indicated in Figures 4-6 along the anterior-posterior axis. Scale bar = 0.1 mm.