- Title
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Genomic, cDNA and embryonic expression analysis of zebrafish IRF6, the gene mutated in the human oral clefting disorders Van der Woude and popliteal pterygium syndromes
- Authors
- Ben, J., Jabs, E.W., and Chong, S.S.
- Source
- Full text @ Gene Expr. Patterns
Whole-mount (A–I) and sagittal section (J–L) analysis of irf6 expression in early zebrafish embryos. Ubiquitous expression is observed in 2-cell (A) and 16-cell (B) cleavage stage embryos, and in the 40% epiboly stage blastula (C). At the 80% epiboly stage (D) and bud stage (E), expression is concentrated in the forerunner cells and Kupffer′s vesicle, respectively. No expression was observed in 6-somite and 10-somite stage embryos (data not shown). At the 14-somite stage, irf6 begins to be expressed in the otic placode (F). At the 22-somite stage, additional expression in the cloaca is observed (G). At 26 hpf, lateral (H) and dorsal (I) views of whole-mounts show expression in olfactory placode, otic vesicle, and gut. Sagittal tissue sections of 26 hpf stained embryos confirm irf6 expression in the olfactory placode (J), otic vesicle (K) and gut (L). No staining signal was observed when embryos and larvae were hybridized with sense RNA probe (data not shown). cl, Cloaca; f, forerunner cell; g, gut; k, Kupffer′s vesicle; of; olfactory placode/vesicle; ot, otic placode/vesicle. |
Expression of irf6 transcript in the developing zebrafish during pharyngula, hatching and larval stages. Whole-mounts of 2 dpf (A) and 3 dpf (B) embryos show strong irf6 expression in the mouth and pharyngeal arch region, with increased expression intensity in 5 dpf larvae (C). Flat-mount lateral (D,E) and ventral (F,G) views, and sagittal section view (H), shows expression in pharyngeal arches at 35 hpf (D), 2 dpf (E), 3 dpf (F), 4 dpf (G), and 5 dpf (H). Sagittal (I,J) and transverse (K,L) tissue sections of stained 2 dpf (I,K), 3 dpf (J), and 5 dpf (H,L) embryos and larvae show localization of irf6 expression in the mouth, pharynx, and gut. Flat-mount dorsal views of digestive system at 35 hpf (M), 2 dpf (N), and 3 dpf (O) reveal irf6 expression in the liver and exocrine pancreas in addition to the esophagus and intestinal bulb (gut). e, Eye; es, esophagus; g, gut; li, liver; m, mouth; nc, notochord; of, olfactory vesicle/naris; ot, otic vesicle; pa, pharyngeal arch; ph, pharynx; np, endocrine pancreas; xp, exocrine pancreas. |
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Reprinted from Gene expression patterns : GEP, 5(5), Ben, J., Jabs, E.W., and Chong, S.S., Genomic, cDNA and embryonic expression analysis of zebrafish IRF6, the gene mutated in the human oral clefting disorders Van der Woude and popliteal pterygium syndromes, 629-638, Copyright (2005) with permission from Elsevier. Full text @ Gene Expr. Patterns