Mapping Details

GENETIC MAPPING PANELS

Chr	Location	Mapped As	Panel	Mapped By	Scoring
2	51.2 cM	z9234	Boston MGH Cross (MGH)	Fishman, Mark C.	Data
2	3029.0 cR	z9234	Goodfellow T51 (T51)	Geisler, Robert	Data
Note: Physical map location as displayed in the genome browsers is more precise than linkage map location. Note: Physical map location should be used whenever possible.

MAPPING FROM PUBLICATIONS

Marker	Type	Chr	Distance	Publication / Person	Comments
z17291	SSLP	2		Bauer et al., 2001	PCR analysis of genomic DNA from laf mutant F2 embryos for SSLP markers put  ... PCR analysis of genomic DNA from laf mutant F2 embryos for SSLP markers put laf^tm110 between the markers z17291 (four recombinations in 192 meioses=2.1 cM) and z9234 (2 recombinations in 192 meioses=1.0 cM) on linkage group 2. No recombination between laf^tm110 and alk8 was found in 280 meioses,
acvr1l	GENE	2		Bauer et al., 2001	PCR analysis of genomic DNA from laf mutant F2 embryos for SSLP markers put  ... PCR analysis of genomic DNA from laf mutant F2 embryos for SSLP markers put laf^tm110 between the markers z17291 (four recombinations in 192 meioses=2.1 cM) and z9234 (2 recombinations in 192 meioses=1.0 cM) on linkage group 2. No recombination between laf^tm110 and alk8 was found in 280 meioses,
m100	Feature	2		Bauer et al., 2001	PCR analysis of genomic DNA from laf mutant F2 embryos for SSLP markers put  ... PCR analysis of genomic DNA from laf mutant F2 embryos for SSLP markers put laf^tm110 between the markers z17291 (four recombinations in 192 meioses=2.1 cM) and z9234 (2 recombinations in 192 meioses=1.0 cM) on linkage group 2. No recombination between laf^tm110 and alk8 was found in 280 meioses,
tm110b	Feature	2	1.0 cM	Bauer et al., 2001	PCR analysis of genomic DNA from laf mutant F2 embryos for SSLP markers put  ... PCR analysis of genomic DNA from laf mutant F2 embryos for SSLP markers put laf^tm110 between the markers z17291 (four recombinations in 192 meioses=2.1 cM) and z9234 (2 recombinations in 192 meioses=1.0 cM) on linkage group 2. No recombination between laf^tm110 and alk8 was found in 280 meioses,

OTHER MAPPING INFORMATION

Primer Sets:

Strain	Bandsize	Restriction Enzyme	Annealing Temperature [C]
AB	0,294		60.0
Forward Primer	TGTGGGAGTGCAGGACAGTA
Reverse Primer	CGCTATGTCAGCTCACTCGA
IND	290		60.0
Forward Primer	TGTGGGAGTGCAGGACAGTA
Reverse Primer	CGCTATGTCAGCTCACTCGA
EKW	0,294		60.0
Forward Primer	TGTGGGAGTGCAGGACAGTA
Reverse Primer	CGCTATGTCAGCTCACTCGA
TU	250		60.0
Forward Primer	TGTGGGAGTGCAGGACAGTA
Reverse Primer	CGCTATGTCAGCTCACTCGA