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Fig. S2 Developmental expansion of midbrain vasculature during 2.0 to 7.5 dpf. Projected confocal images (left) and 3-D reconstruction (middle, right) of a larva′s midbrain vasculature imaged at 2.0, 2.5, 3.0, 3.5, 4.5, and 7.5 dpf. The dashed square delineates the midbrain position. The segments that were pruned at the next time point are marked in red (middle). The newly formed segments after 2.0 dpf through angiogenesis are marked in green (right). Yellow, BCA; white, midbrain vasculature; blue, CVP. Scale, 50 μm.
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