Transgenic Construct

Tg3(Xla.Rho:crb2a-HA)

ID

ZDB-TGCONSTRCT-110131-38

Previous Names

IntraWT (1)
Tg(Xla.Rho:crb2.3-HA) (1)
Tg(Xla.Rho:crb2a.3-HA)

Type

engineered_plasmid

Regulatory Regions

None

Coding Sequences

crb2a (1)
HA (1)

Contains Sequences

HA (1)

Note

This construct utilizes 0.8 kB of the Xenopus rod opsin (Rho) promoter with an N terminal signal peptide takenfrom zebrafish Crb2b and an influenza hamagglutinin (HA) tag (YPYDVPDYA). To make IntraWt, the SP from zebrafish Crb2b was introduced with PCR using a pair of primers in which the HA tag sequence was engineered immediately after SP within the reverse primer and the sequence containing the Crb2a transmembrane domain plus the intracellular domain was cloned in-frame following the HA sequence.

Plasmid Map

None

Genomic Features

That Utilize Tg3(Xla.Rho:crb2a-HA) Construct

Genomic Feature	Affected Genomic Regions
umz10Tg
umz11Tg
umz12Tg

Transgenics

That Utilize Tg3(Xla.Rho:crb2a-HA) Construct

Fish	Affected Genomic Regions	Gene Expression
slc45a2^b4/+; fl1Tg/+; umz10Tg/+	slc45a2
slc45a2^b4/+; fl1Tg/+; umz11Tg/+	slc45a2
slc45a2^b4/b4; fl1Tg; umz10Tg	slc45a2	5 figures from Hsu et al., 2010
slc45a2^b4/b4; fl1Tg; umz11Tg	slc45a2
slc45a2^b4/b4; fl1Tg; umz12Tg	slc45a2
umz12Tg

Sequence Information

Other Construct Pages

None

Citations