PUBLICATION
Development of noradrenergic neurons in the zebrafish hindbrain requires BMP, FGF8, and the homeodomain protein Soulless/Phox2a
- Authors
- Guo, S., Brush, J., Teraoka, H., Goddard, A., Wilson, S.W., Mullins, M.C., and Rosenthal, A.
- ID
- ZDB-PUB-991129-3
- Date
- 1999
- Source
- Neuron 24: 555 (Journal)
- Registered Authors
- Guo, Su, Mullins, Mary C., Teraoka, Hiroki, Wilson, Steve
- Keywords
- none
- MeSH Terms
-
- Animals
- Nerve Tissue Proteins
- Zebrafish/embryology
- Fibroblast Growth Factors/physiology*
- Molecular Sequence Data
- Tyrosine 3-Monooxygenase/metabolism
- Dopamine beta-Hydroxylase/metabolism
- Neurons/metabolism
- Neurons/physiology*
- Bone Morphogenetic Proteins/physiology*
- Embryo, Nonmammalian/cytology
- Embryo, Nonmammalian/physiology
- Fibroblast Growth Factor 8
- Norepinephrine/physiology*
- Homeodomain Proteins/physiology*
- Zebrafish Proteins/physiology*
- Locus Coeruleus/embryology
- Stem Cells/metabolism
- Sequence Homology, Amino Acid
- Amino Acid Sequence/genetics
- Transcription Factors/physiology*
- Rhombencephalon/embryology*
- PubMed
- 10595509 Full text @ Neuron
Citation
Guo, S., Brush, J., Teraoka, H., Goddard, A., Wilson, S.W., Mullins, M.C., and Rosenthal, A. (1999) Development of noradrenergic neurons in the zebrafish hindbrain requires BMP, FGF8, and the homeodomain protein Soulless/Phox2a. Neuron. 24:555.
Abstract
We report that the zebrafish mutation soulless, in which the development of locus coeruleus (LC) noradrenergic (NA) neurons failed to occur, disrupts the homeodomain protein Phox2a. Phox2a is not only necessary but also sufficient to induce Phox2b+ dopamine-beta-hydroxylase+ and tyrosine hydroxylase+ NA neurons in ectopic locations. Phox2a is first detected in LC progenitors in the dorsal anterior hindbrain, and its expression there is dependent on FGF8 from the mid/hindbrain boundary and on optimal concentrations of BMP signal from the epidermal ectoderm/future dorsal neural plate junction. These findings suggest that Phox2a coordinates the specification of LC in part through the induction of Phox2b and in response to cooperating signals that operate along the mediolateral and anteroposterior axes of the neural plate.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping