PUBLICATION
Analysis of a 26-kb region linked to the MHC in zebrafish: genomic organization of the proteasome component beta/transporter associated with antigen processing-2 gene cluster and identification of five new proteasome beta subunit genes
- Authors
- Murray, B.W., Sültmann, H., and Klein, J.
- ID
- ZDB-PUB-990824-17
- Date
- 1999
- Source
- Journal of immunology (Baltimore, Md. : 1950) 163(5): 2657-2666 (Journal)
- Registered Authors
- Klein, Jan, Sültmann, Holger
- Keywords
- none
- MeSH Terms
-
- ATP-Binding Cassette Transporters/chemistry
- ATP-Binding Cassette Transporters/genetics*
- Alleles
- Amino Acid Sequence
- Animals
- Antigen Presentation/genetics*
- Base Composition/immunology
- Base Sequence
- Contig Mapping
- Cysteine Endopeptidases/chemistry
- Cysteine Endopeptidases/genetics*
- Endopeptidases/chemistry
- Endopeptidases/genetics
- Endopeptidases/isolation & purification
- Exons/immunology
- Introns/immunology
- Major Histocompatibility Complex/genetics*
- Molecular Sequence Data
- Multienzyme Complexes/chemistry
- Multienzyme Complexes/genetics*
- Multigene Family/immunology*
- Phylogeny
- Promoter Regions, Genetic/immunology
- Proteasome Endopeptidase Complex
- Zebrafish/genetics*
- Zebrafish/immunology*
- Zebrafish Proteins*
- PubMed
- 10453006
Citation
Murray, B.W., Sültmann, H., and Klein, J. (1999) Analysis of a 26-kb region linked to the MHC in zebrafish: genomic organization of the proteasome component beta/transporter associated with antigen processing-2 gene cluster and identification of five new proteasome beta subunit genes. Journal of immunology (Baltimore, Md. : 1950). 163(5):2657-2666.
Abstract
Sequencing of zebrafish (Danio rerio) bacterial artificial chromosome and P1 artificial chromosome genomic clone fragments and of cDNA clones has led to the identification of five new loci coding for beta subunits of proteasomes (PSMB). Together with the four genes identified previously, nine PSMB genes have now been defined in the zebrafish. Six of the nine genes reside in the zebrafish MHC (Mhc) class I region, four of them reside in a single cluster closely associated with TAP2 on a 26-kb long genomic fragment, and two reside at some distance from the fragment. In addition to homologues of the human genes PSMB5 through PSMB9, two new genes, PSMB11 and PSMB12, have been found for which there are no known corresponding genes in humans. The new genes reside in the PSMB cluster in the Mhc. Homology and promoter region analysis suggest that the Mhc-associated genes might be inducible by IFN-gamma. The zebrafish class I region contains representatives of three phylogenetically distinguishable groups of PSMB genes, X, Y, and Z. It is proposed that these genes were present in the ancestral PSMB region before Mhc class I genes became associated with it.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping