PUBLICATION
Mutations in the zebrafish unmask shared regulatory pathways controlling the development of catecholaminergic neurons
- Authors
- Guo, S., Wilson, S.W., Cooke, S., Chitnis, A.B., Driever, W., and Rosenthal, A.
- ID
- ZDB-PUB-990416-2
- Date
- 1999
- Source
- Developmental Biology 208: 473-487 (Journal)
- Registered Authors
- Chitnis, Ajay, Driever, Wolfgang, Guo, Su, Wilson, Steve
- Keywords
- none
- MeSH Terms
-
- Animals
- Branchial Region/abnormalities
- Cell Differentiation
- Central Nervous System/cytology
- Central Nervous System/embryology
- Eye/cytology
- Eye/embryology
- Genes, Regulator
- Genetic Complementation Test
- Hypothalamus/cytology
- Hypothalamus/embryology
- Immunohistochemistry
- In Situ Hybridization
- Locus Coeruleus/cytology
- Locus Coeruleus/embryology
- Mutation
- Nervous System/embryology*
- Neurons/cytology*
- Peripheral Nervous System/cytology
- Peripheral Nervous System/embryology
- Prosencephalon/cytology
- Prosencephalon/embryology
- Receptors, Adrenergic/isolation & purification
- Receptors, Catecholamine/isolation & purification*
- Receptors, Dopamine/isolation & purification
- Rhombencephalon/cytology
- Rhombencephalon/embryology
- Telencephalon/cytology
- Telencephalon/embryology
- Zebrafish/embryology*
- Zebrafish/genetics*
- PubMed
- 10191060 Full text @ Dev. Biol.
Citation
Guo, S., Wilson, S.W., Cooke, S., Chitnis, A.B., Driever, W., and Rosenthal, A. (1999) Mutations in the zebrafish unmask shared regulatory pathways controlling the development of catecholaminergic neurons. Developmental Biology. 208:473-487.
Abstract
The mechanism by which pluripotent progenitors give rise to distinct classes of mature neurons in vertebrates is not well understood. To address this issue we undertook a genetic screen for mutations which affect the commitment and differentiation of catecholaminergic (CA) [dopaminergic (DA), noradrenergic (NA), and adrenergic] neurons in the zebrafish, Danio rerio. The identified mutations constitute five complementation groups. motionless and foggy affect the number and differentiation state of hypothalamic DA, telencephalic DA, retinal DA, locus coeruleus (LC) NA, and sympathetic NA neurons. The too few mutation leads to a specific reduction in the number of hypothalamic DA neurons. no soul lacks arch-associated NA cells and has defects in pharyngeal arches, and soulless lacks both arch-associated and LC cell groups. Our analyses suggest that the genes defined by these mutations regulate different steps in the differentiation of multipotent CA progenitors. They further reveal an underlying universal mechanism for the control of CA cell fates,which involve combinatorial usage of regulatory genes.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping