PUBLICATION
Role of sonic hedgehog in branchiomotor neuron induction in zebrafish
- Authors
- Chandrasekhar, A., Warren, Jr., J.T., Takahashi, K., Schauerte, H.E., van Eeden, F.J.M., Haffter, Pl, and Kuwada, J.Y.
- ID
- ZDB-PUB-981105-2
- Date
- 1998
- Source
- Mechanisms of Development 76: 101-115 (Journal)
- Registered Authors
- Chandrasekhar, Anand, Haffter, Pascal, Kuwada, John, Schauerte, Heike, van Eeden, Freek, Warren, James T., Jr.
- Keywords
- zebrafish; hindbrain; rhombomere; branchiomotor neuron; spinal cord; cyclops; sonic-you; sonic hedgehog; tiggy-winkle hedgehog
- MeSH Terms
-
- Trans-Activators*
- Embryonic Induction
- Motor Neurons/physiology
- Neurons/physiology*
- Branchial Region/innervation
- Zebrafish/embryology*
- Cell Nucleus/pathology
- Spinal Cord
- Hedgehog Proteins
- Nervous System/embryology
- Zebrafish Proteins
- Transforming Growth Factor beta/genetics
- Embryo, Nonmammalian
- Animals
- Rhombencephalon/embryology
- Rhombencephalon/pathology
- Gene Expression Regulation, Developmental*
- Intracellular Signaling Peptides and Proteins
- Mutation
- Proteins/genetics*
- Proteins/metabolism
- PubMed
- 9767138 Full text @ Mech. Dev.
Citation
Chandrasekhar, A., Warren, Jr., J.T., Takahashi, K., Schauerte, H.E., van Eeden, F.J.M., Haffter, Pl, and Kuwada, J.Y. (1998) Role of sonic hedgehog in branchiomotor neuron induction in zebrafish. Mechanisms of Development. 76:101-115.
Abstract
The role of zebrafish hedgehog genes in branchiomotor neuron development was analyzed by examining mutations that affect the expression of the hedgehog genes and by overexpressing these genes in embryos. In cyclops mutants, reduction in sonic hedgehog (shh) expression, and elimination of tiggy-winkle hedgehog (twhh) expression, correlated with reductions in branchiomotor neuron populations. Furthermore, branchiomotor neurons were restored in cyclops mutants when shh or twhh was overexpressed. These results suggest that Shh and/or Twhh play an important role in the induction of branchiomotor neurons in vivo. In sonic-you (syu) mutants, where Shh activity was reduced or eliminated due to mutations in shh, branchiomotor neurons were reduced in number in a rhombomere-specific fashion, but never eliminated. Similarly, spinal motor neurons were reduced, but not eliminated, in syu mutants. These results demonstrate that Shh is not solely responsible for inducing branchiomotor and spinal motor neurons, and suggest that Shh and Twhh may function as partially redundant signals for motor neuron induction in zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping