PUBLICATION
The zebrafish Pax3 and Pax7 homologues are highly conserved, encode multiple isoforms and show dynamic segment-like expression in the developing brain
- Authors
- Seo, H.C., Sætre, B.O., Havik, B., Ellingsen, S., and Fjose, A.
- ID
- ZDB-PUB-980319-2
- Date
- 1998
- Source
- Mechanisms of Development 70: 49-63 (Journal)
- Registered Authors
- Ellingsen, Ståle, Fjose, Anders, Havik, Bjarte, Seo, Hee-Chan
- Keywords
- alternative splicing; hindbrain segmentation; homeodomain; paired domain
- MeSH Terms
-
- Zebrafish/embryology*
- Zebrafish/genetics*
- Homeodomain Proteins*
- Somites/metabolism
- Transcription Factors*
- Molecular Sequence Data
- Mesoderm/metabolism
- Animals
- Nerve Tissue Proteins/genetics*
- Paired Box Transcription Factors
- Gene Expression Regulation, Developmental
- DNA, Complementary/genetics
- Zebrafish Proteins*
- In Situ Hybridization
- Mammals
- PAX7 Transcription Factor
- DNA Primers/genetics
- Sequence Homology, Amino Acid
- Base Sequence
- Amino Acid Sequence
- Brain/embryology*
- Species Specificity
- Conserved Sequence
- DNA-Binding Proteins/genetics*
- Muscle Proteins/genetics*
- PubMed
- 9510024 Full text @ Mech. Dev.
Citation
Seo, H.C., Sætre, B.O., Havik, B., Ellingsen, S., and Fjose, A. (1998) The zebrafish Pax3 and Pax7 homologues are highly conserved, encode multiple isoforms and show dynamic segment-like expression in the developing brain. Mechanisms of Development. 70:49-63.
Abstract
This study describes the isolation and characterization of zebrafish homologues of the mammalian Pax3 and Pax7 genes. The proteins encoded by both zebrafish genes are highly conserved (>83%) relative to the known mammalian sequences. Also the neural expression patterns during embryogenesis are very similar to the murine homologues. However, observed differences in neural crest and mesodermal expression relative to mammals could reflect some functional divergence in the development of these tissues. For the zebrafish Pax7 protein we report the first full-length amino acid sequences in vertebrates and show the existence of three additional isoforms which have truncations in the homeodomain and/or the C-terminal region. These novel variants provide evidence for additional isoform diversity of vertebrate Pax proteins.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping