PUBLICATION
Overexpression of neurogenin induces ectopic expression of HuC in zebrafish
- Authors
- Kim, C.-H., Y.-K. Bae, Y. Yamanaka, S. Yamashita, T. Shimizu, R. Fujii, H.-C. Park, S.-Y. Yeo, T.-L. Huh, M. Hibi, and T. Hirano
- ID
- ZDB-PUB-980112-2
- Date
- 1997
- Source
- Neuroscience letters 239: 113-116 (Journal)
- Registered Authors
- Bae, Young Ki, Fujii, Ritsuko, Hibi, Masahiko, Huh, Tae-Lin, Kim, Cheol-Hee, Shimizu, Takashi, Yamanaka, Yojiro, Yamashita, Susumu
- Keywords
- zebrafish; neurogenin; ZATH-1; HuC; neuronal determination; neural plate
- MeSH Terms
-
- Brain/embryology
- Brain/metabolism*
- Molecular Sequence Data
- ELAV-Like Protein 3
- Biomarkers/analysis
- RNA-Binding Proteins*
- Basic Helix-Loop-Helix Transcription Factors
- In Situ Hybridization
- Zebrafish/embryology*
- Xenopus Proteins*
- Antigens, Surface/metabolism*
- Gene Expression Regulation, Developmental*
- Nervous System/embryology
- Nervous System/metabolism*
- Amino Acid Sequence
- Animals
- Zebrafish Proteins*
- ELAV Proteins
- Nerve Tissue Proteins/metabolism*
- Nerve Tissue Proteins/physiology*
- RNA, Messenger/analysis
- RNA, Messenger/genetics
- PubMed
- 9469669 Full text @ Neurosci. Lett.
Citation
Kim, C.-H., Y.-K. Bae, Y. Yamanaka, S. Yamashita, T. Shimizu, R. Fujii, H.-C. Park, S.-Y. Yeo, T.-L. Huh, M. Hibi, and T. Hirano (1997) Overexpression of neurogenin induces ectopic expression of HuC in zebrafish. Neuroscience letters. 239:113-116.
Abstract
Several basic helix-loop-helix (bHLH) transcription factors are known to be involved in vertebrate neurogenesis. To investigate their roles in zebrafish neurogenesis, we isolated cDNAs for homologues of neurogenin and Math(-1)/atonal. The transcription of neurogenin was first detectable in zebrafish nervous system at late gastrulation stage. The expression of zebrafish neurogenin precedes and overlaps that of HuC, one of the earliest neuronal precursor markers. Injection of neurogenin mRNA into early stage zebrafish embryos induced ectopic expression of HuC. These results suggest that neurogenin may participate in the generation of HuC-expressing cells, implying its role in neuronal determination in zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping