PUBLICATION

Positive and Negative Regulation of Muscle Cell Identity by Members of the hedgehog and TGF-β Gene Families

Authors
Du, S.J., S.H. Devoto, M. Westerfield, and R.T. Moon
ID
ZDB-PUB-971016-36
Date
1997
Source
The Journal of cell biology   139(1): 145-156 (Journal)
Registered Authors
Devoto, Stephen Henri, Du, Shao Jun (Jim), Moon, Randall T., Westerfield, Monte
Keywords
none
MeSH Terms
  • Animals
  • Cell Differentiation
  • Cyclic AMP-Dependent Protein Kinases/antagonists & inhibitors
  • Cyclic AMP-Dependent Protein Kinases/physiology
  • Down-Regulation/genetics*
  • Drosophila
  • Drosophila Proteins*
  • Embryo, Nonmammalian
  • Gene Expression Regulation, Developmental*
  • Hedgehog Proteins
  • Insect Proteins/biosynthesis
  • Insect Proteins/genetics*
  • Insect Proteins/physiology
  • Multigene Family
  • Muscle Fibers, Skeletal/cytology
  • Muscle Fibers, Skeletal/enzymology
  • Muscle Fibers, Skeletal/physiology*
  • Muscle Fibers, Slow-Twitch/cytology
  • Muscle Fibers, Slow-Twitch/physiology
  • Notochord/metabolism
  • Signal Transduction
  • Transforming Growth Factor beta/genetics*
  • Transforming Growth Factor beta/physiology
  • Transforming Growth Factors/biosynthesis
  • Transforming Growth Factors/physiology
  • Up-Regulation/genetics*
  • Zebrafish
  • Zebrafish Proteins*
PubMed
9314535 Full text @ J. Cell Biol.
Abstract
We have examined whether the development of embryonic muscle fiber type is regulated by competing influences between Hedgehog and TGF-beta signals, as previously shown for development of neuronal cell identity in the neural tube. We found that ectopic expression of Hedgehogs or inhibition of protein kinase A in zebrafish embryos induces slow muscle precursors throughout the somite but muscle pioneer cells only in the middle of the somite. Ectopic expression in the notochord of Dorsalin-1, a member of the TGF-beta superfamily, inhibits the formation of muscle pioneer cells, demonstrating that TGF-beta signals can antagonize the induction of muscle pioneer cells by Hedgehog. We propose that a Hedgehog signal first induces the formation of slow muscle precursor cells, and subsequent Hedgehog and TGF-beta signals exert competing positive and negative influences on the development of muscle pioneer cells.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping