PUBLICATION
Development of branchiomotor neurons in zebrafish
- Authors
- Chandrasekhar, A., Moens, C.B., Warren, J.T., Kimmel, C.B., and Kuwada, J.Y.
- ID
- ZDB-PUB-970812-16
- Date
- 1997
- Source
- Development (Cambridge, England) 124(13): 2633-2644 (Journal)
- Registered Authors
- Chandrasekhar, Anand, Kimmel, Charles B., Kuwada, John, Moens, Cecilia, Warren, James T., Jr.
- Keywords
- zebrafish; hindbrain; branchiomotor neuron; axonogenesis; valentino; cyclops
- MeSH Terms
-
- Homeodomain Proteins/biosynthesis
- Homeodomain Proteins/genetics
- Avian Proteins*
- Oncogene Proteins*
- Contactin 2
- Neural Pathways/embryology*
- Zebrafish/embryology
- Zebrafish Proteins
- Embryonic Induction
- Cell Movement
- Cell Adhesion Molecules, Neuronal/biosynthesis
- Cell Adhesion Molecules, Neuronal/genetics
- Gene Expression
- Neural Tube Defects/genetics
- Maf Transcription Factors
- Branchial Region/embryology*
- Branchial Region/innervation*
- Rhombencephalon/cytology
- Rhombencephalon/embryology*
- MafB Transcription Factor
- Mutation
- Motor Neurons*
- DNA-Binding Proteins/biosynthesis
- DNA-Binding Proteins/genetics
- Nerve Tissue Proteins/biosynthesis
- Nerve Tissue Proteins/genetics
- Animals
- Transcription Factors/biosynthesis
- Transcription Factors/genetics
- Body Patterning
- PubMed
- 9217005 Full text @ Development
Citation
Chandrasekhar, A., Moens, C.B., Warren, J.T., Kimmel, C.B., and Kuwada, J.Y. (1997) Development of branchiomotor neurons in zebrafish. Development (Cambridge, England). 124(13):2633-2644.
Abstract
The mechanisms underlying neuronal specification and axonogenesis in the vertebrate hindbrain are poorly understood. To address these questions, we have employed anatomical methods and mutational analysis to characterize the branchiomotor neurons in the zebrafish embryo. The zebrafish branchiomotor system is similar to those in the chick and mouse, except for the location of the nVII and nIX branchiomotor neurons. Developmental analyses of genes expressed by branchiomotor neurons suggest that the different location of the nVII neurons in the zebrafish may result from cell migration. To gain insight into the mechanisms underlying the organization and axonogenesis of these neurons, we examined the development of the branchiomotor pathways in neuronal mutants. The valentino b337 mutation blocks the formation of rhombomeres 5 and 6, and severely affects the development of the nVII and nIX motor nuclei. The cyclops b16 mutation deletes ventral midline cells in the neural tube, and leads to a severe disruption of most branchiomotor nuclei and axon pathways. These results demonstrate that rhombomere- specific cues and ventral midline cells play important roles in the development of the branchiomotor pathways.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping