PUBLICATION

Regulatory gene expression boundaries demarcate sites of neuronal differentiation in the embryonic zebrafish forebrain

Authors
Macdonald, R., Xu, Q., Barth, K.A., Mikkola, I., Holder, N., Fjose, A., Krauss, S., and Wilson, S.W.
ID
ZDB-PUB-961014-719
Date
1994
Source
Neuron   13: 1039-1053 (Journal)
Registered Authors
Barth, Anukampa, Fjose, Anders, Holder, Nigel, Krauss, Stefan, Macdonald, Rachel, Wilson, Steve, Xu, Qiling
Keywords
none
MeSH Terms
  • Neurons/cytology*
  • Prosencephalon/embryology*
  • DNA-Binding Proteins/genetics
  • Repressor Proteins
  • Forkhead Transcription Factors
  • Wnt Proteins
  • Receptor Protein-Tyrosine Kinases/genetics
  • Cell Differentiation
  • Transcription Factors/genetics
  • PAX2 Transcription Factor
  • Eye Proteins
  • Proto-Oncogene Proteins/genetics
  • Zebrafish Proteins*
  • Homeodomain Proteins*
  • Animals
  • Genes, Regulator
  • Morphogenesis
  • Zebrafish
  • Paired Box Transcription Factors
  • Neural Pathways/embryology
  • In Situ Hybridization
  • Gene Expression Regulation, Developmental*
PubMed
7946344 Full text @ Neuron
Abstract
During development of the zebrafish forebrain, a simple scaffold of axon pathways is pioneered by a small number of neurons. We show that boundaries of expression domains of members of the eph, forkhead, pax, and wnt gene families correlate with the positions at which these neurons differentiate and extend axons. Analysis of genetically or experimentally altered forebrains indicates that if a boundary is maintained, there is appropriate neural differentiation with respect to the boundary. Conversely, in the absence of a boundary, there is concomitant disruption of neural patterning. We also show that a strip of cells within the dorsal diencephalon shares features with ventral midline cells. This strip of cells fails to develop in mutant fish in which specification of the ventral CNS is disrupted, suggesting that its development may be regulated by the same inductive pathways that pattern the ventral midline.
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping