PUBLICATION
Expression of a type II collagen gene in the zebrafish embryonic axis
- Authors
- Yan, Y.L., Hatta, K., Riggleman, B., and Postlethwait, J.H.
- ID
- ZDB-PUB-961014-1290
- Date
- 1995
- Source
- Developmental Dynamics : an official publication of the American Association of Anatomists 203: 363-376 (Journal)
- Registered Authors
- Hatta, Kohei, Postlethwait, John H., Riggleman, Bob, Yan, Yi-Lin
- Keywords
- Type II collagen, Axis specification, Zabrafish, Danio rerio, Axial, Hedgehog, Twist, Hypochord, Floor plate, Notochord, COL2A1
- MeSH Terms
-
- Animals
- Base Sequence
- Collagen/genetics*
- Embryo, Nonmammalian/physiology*
- Gene Expression/physiology*
- Gene Expression Regulation, Developmental/physiology
- Head/embryology
- Immunohistochemistry
- In Situ Hybridization
- Limb Buds/embryology
- Mesoderm/physiology
- Molecular Sequence Data
- Morphogenesis/physiology
- Mutation/physiology
- Notochord/physiology
- Sequence Homology, Amino Acid
- Zebrafish/embryology*
- PubMed
- 8589433 Full text @ Dev. Dyn.
Citation
Yan, Y.L., Hatta, K., Riggleman, B., and Postlethwait, J.H. (1995) Expression of a type II collagen gene in the zebrafish embryonic axis. Developmental Dynamics : an official publication of the American Association of Anatomists. 203:363-376.
Abstract
To understand the hierarchy of developmental controls underlying axis specification in vertebrate embryos, it is helpful to identify relationships between regulatory molecules and the genes that give axial cells their differentiated phenotypes. This work reports the cloning and expression pattern of one of these differentiation genes, a type II collagen (col2a1) gene from the zebrafish Danio rerio. Along the embryonic axis, col2a1 is expressed dynamically in three rows that are each a single cell wide: the notochord and the rows of cells immediately dorsal and ventral to it--the floor plate of the central nervous system, and the hypochord. In addition, col2a1 is expressed in the pharyngeal arches, the epithelium of the otic capsule, and in the mesenchyme of the neurocranium. Experiments probed the expression pattern of col2a1 relative to that of known or potential regulators of axis development, including axial, sonic hedgehog, twist, and cyclops. The results showed that the spatial and temporal pattern of col2a1 expression in axial mesoderm follows the expression of twist closer than other genes tested. In cyclops embryos, which lack an intact floor plate, col2a1 expression was usually low, but not missing in cells in the ventral spinal cord. Because col2a1 expression reveals abnormalities in the notochord of cyclopsb16 embryos, and less col2a1-expressing mesenchyme accumulates rostral to the notochord in cyclops embryos, the effects of the cyclopsb16 mutation are not confined to the central nervous system.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping