PUBLICATION
Fibronectin and Hand2 influence tubulogenesis during pronephros development and mesonephros regeneration in zebrafish (Danio rerio)
- Authors
- Uribe-Montes, L.C., Sanabria-Camargo, C.A., Piñeros-Romero, C.C., Otálora-Tarazona, S., Ávila-Jiménez, E., Acosta-Virgüez, E., Garavito-Aguilar, Z.V.
- ID
- ZDB-PUB-240907-9
- Date
- 2024
- Source
- PLoS One 19: e0307390e0307390 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Animals
- Basic Helix-Loop-Helix Transcription Factors/genetics
- Basic Helix-Loop-Helix Transcription Factors/metabolism
- Fibronectins*/genetics
- Fibronectins*/metabolism
- Gene Expression Regulation, Developmental
- Kidney Tubules/metabolism
- Mesonephros/metabolism
- Mutation
- Organogenesis/genetics
- Pronephros*/embryology
- Pronephros*/metabolism
- Regeneration*
- Zebrafish*/genetics
- Zebrafish Proteins*/genetics
- Zebrafish Proteins*/metabolism
- PubMed
- 39240899 Full text @ PLoS One
Citation
Uribe-Montes, L.C., Sanabria-Camargo, C.A., Piñeros-Romero, C.C., Otálora-Tarazona, S., Ávila-Jiménez, E., Acosta-Virgüez, E., Garavito-Aguilar, Z.V. (2024) Fibronectin and Hand2 influence tubulogenesis during pronephros development and mesonephros regeneration in zebrafish (Danio rerio). PLoS One. 19:e0307390e0307390.
Abstract
Worldwide incidence of kidney diseases has been rising. Thus, recent research has focused on zebrafish, whose fast development and innate regeneration capacity allow identifying factors influencing renal processes. Among these poorly studied factors are extracellular matrix (ECM) proteins like Fibronectin (Fn) essential in various tissues but not yet evaluated in a renal context. We utilized early nat and han zebrafish mutant embryos and carrier adults to investigate Fn's role during kidney development and regeneration. The locus natter (nat) encodes Fn and the locus han encodes Hand2, which results in increased Fn deposition. Our results show that Fn impacts identity maintenance and morphogenesis during development and influences conditions for neonephrogenic cluster formation during regeneration. Histological analysis revealed disrupted pronephric structures and increased blood cell accumulation in Fn mutants. Despite normal expression of specification markers (pax2, ATPα1a.1), structural abnormalities were evident. Differences between wild-type and mutation-carriers suggest a haploinsufficiency scenario. These findings reveal a novel function for ECM in renal development and regeneration, with potential implications for understanding and treating kidney diseases.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping