PUBLICATION
Effect of caldesmon mutations in the development of zebrafish embryos
- Authors
- Virtanen, V., Paunu, K., Niva, S., Sundvall, M., Paatero, I.
- ID
- ZDB-PUB-230602-32
- Date
- 2023
- Source
- Biochemical and Biophysical Research Communications 669: 101810-18 (Journal)
- Registered Authors
- Paatero, Ilkka
- Keywords
- Caldesmon, Developmental biology, Mutation, Zebrafish
- MeSH Terms
-
- Animals
- Calmodulin-Binding Proteins*/genetics
- Mutation
- Zebrafish*/metabolism
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- PubMed
- 37262948 Full text @ Biochem. Biophys. Res. Commun.
Citation
Virtanen, V., Paunu, K., Niva, S., Sundvall, M., Paatero, I. (2023) Effect of caldesmon mutations in the development of zebrafish embryos. Biochemical and Biophysical Research Communications. 669:101810-18.
Abstract
Cancer is a profound medical concern and better treatments are needed for cancer patients. Therefore, new cancer targets are constantly being studied. These targets need not only be relevant for cancer progression, but their modulation needs to be tolerated reasonably well by the host. Caldesmon is one of these proposed novel targets for cancer therapy. Therefore, we analyzed effects of caldesmon mutations in normal development using genetically modified zebrafish embryos. We analyzed mutations in both zebrafish caldesmon genes, cald1a and cald1b and analyzed effects of either mutation alone or as in combination in double homozygous embryos using molecular, morphological and functional analyses. The effects of caldesmon mutations were mild and the gross development of zebrafish embryos was normal. The caldesmon mutant embryos had, however, alterations in response to light-stimulus in behavioural assays. Taken together, the effects of caldesmon mutations in the development of zebrafish embryos were reasonably well tolerated and did not indicate significant concerns for caldesmon being a potential target for cancer therapy.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping