PUBLICATION
Foxm1 regulates cardiomyocyte proliferation in adult zebrafish after cardiac injury
- Authors
- Zuppo, D.A., Missinato, M.A., Santana-Santos, L., Li, G., Benos, P.V., Tsang, M.
- ID
- ZDB-PUB-230228-37
- Date
- 2023
- Source
- Development (Cambridge, England) 150(6): (Journal)
- Registered Authors
- Tsang, Michael
- Keywords
- Binucleation, Cardiomyocyte proliferation, Cenpf, Foxm1, Heart regeneration, Zebrafish
- Datasets
- GEO:GSE201139
- MeSH Terms
-
- Animals
- Cell Proliferation/genetics
- Forkhead Box Protein M1/genetics
- Heart/physiology
- Heart Injuries*
- Mammals
- Myocytes, Cardiac*/metabolism
- Zebrafish/genetics
- PubMed
- 36846912 Full text @ Development
Citation
Zuppo, D.A., Missinato, M.A., Santana-Santos, L., Li, G., Benos, P.V., Tsang, M. (2023) Foxm1 regulates cardiomyocyte proliferation in adult zebrafish after cardiac injury. Development (Cambridge, England). 150(6):.
Abstract
The regenerative capacity of the mammalian heart is poor with one potential reason being that adult cardiomyocytes cannot proliferate at sufficient levels to replace lost tissue. During development and neonatal stages, cardiomyocytes can successfully divide under injury conditions; however, as these cells mature their ability to proliferate is lost. Therefore, understanding regulatory programs that can induce post-mitotic cardiomyocytes into a proliferative state is essential to enhance cardiac regeneration. Here we report the forkhead transcription factor, foxm1, is required for cardiomyocyte proliferation after injury through transcriptional regulation of cell cycle genes. Transcriptomic analysis of injured zebrafish hearts revealed that foxm1 expression is increased in border zone cardiomyocytes. Decreased cardiomyocyte proliferation and expression of cell cycle genes in foxm1 mutant hearts was observed, suggesting it is required for cell cycle checkpoints. Subsequent analysis of a candidate Foxm1 target gene, cenpf, revealed this microtubule and kinetochore binding protein is also required for cardiac regeneration. Moreover, cenpf mutants show increased cardiomyocyte binucleation. Thus, foxm1 and cenpf are required for cardiomyocytes to complete mitosis during zebrafish cardiac regeneration.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping