PUBLICATION
Evaluation of a Rapid and Simple Method for Assessing Retinal Vessel Structures in Adult Zebrafish
- Authors
- Lee, Y.R., Son, M., Kim, Y.S., Kim, J.S., Kim, C.H., Jung, S.H.
- ID
- ZDB-PUB-221212-8
- Date
- 2022
- Source
- International Journal of Molecular Sciences 23(23): (Journal)
- Registered Authors
- Jung, Seung-Hyun, Kim, Cheol-Hee
- Keywords
- blood–retinal barrier, hyperglycemia, retinal vasculature, tight junction, trypsin digestion, zebrafish
- MeSH Terms
-
- Animals
- Blood-Retinal Barrier
- Endothelial Cells
- Retinal Diseases*/metabolism
- Retinal Vessels/metabolism
- Trypsin/metabolism
- Zebrafish*
- Zebrafish Proteins/metabolism
- PubMed
- 36499406 Full text @ Int. J. Mol. Sci.
Citation
Lee, Y.R., Son, M., Kim, Y.S., Kim, J.S., Kim, C.H., Jung, S.H. (2022) Evaluation of a Rapid and Simple Method for Assessing Retinal Vessel Structures in Adult Zebrafish. International Journal of Molecular Sciences. 23(23):.
Abstract
The evaluation of retinal vascular structures is important for analyzing various ophthalmic diseases. Conventional trypsin digestion was used for separating retinal vasculatures in mouse, rat, and other animal models; however, the trypsin method alone is technically difficult to perform and has not been reported in zebrafish to date. In this study, we introduced a rapid and convenient method that allows the investigation of fine vessel structures at a cellular level in the relatively intact retinal vasculature of adult zebrafish. Using an anti-ZO-1 antibody, tight junction structures in retinal vessels were examined in detail and several different cell types constituting blood vessels in arterial and capillary areas were identified. In addition, using cell type-specific antibodies, we identified smooth muscle cells, blood cells, and endothelial cells in the retinal vasculature. Finally, using the hyperglycemic model, we observed the dilation of retinal vessels, the downregulation of tight junction proteins, and the reduction in smooth muscle cells. Based on these results, we provide a rapid and convenient method for the study of retinal vasculature disease in the zebrafish animal model.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping