PUBLICATION
A cAMP Sensor Based on Ligand-Dependent Protein Stabilization
- Authors
- Sidoli, M., Chen, L.C., Lu, A.J., Wandless, T.J., Talbot, W.S.
- ID
- ZDB-PUB-220716-8
- Date
- 2022
- Source
- ACS Chemical Biology 17(8): 2024-2030 (Journal)
- Registered Authors
- Talbot, William S.
- Keywords
- none
- MeSH Terms
-
- Animals
- Biosensing Techniques*
- Cyclic AMP*/metabolism
- Cyclic AMP-Dependent Protein Kinases/metabolism
- Fluorescence Resonance Energy Transfer
- Ligands
- Zebrafish/metabolism
- PubMed
- 35839076 Full text @ ACS Chem. Biol.
Citation
Sidoli, M., Chen, L.C., Lu, A.J., Wandless, T.J., Talbot, W.S. (2022) A cAMP Sensor Based on Ligand-Dependent Protein Stabilization. ACS Chemical Biology. 17(8):2024-2030.
Abstract
cAMP is a ubiquitous second messenger with many functions in diverse organisms. Current cAMP sensors, including Föster resonance energy transfer (FRET)-based and single-wavelength-based sensors, allow for real time visualization of this small molecule in cultured cells and in some cases in vivo. Nonetheless the observation of cAMP in living animals is still difficult, typically requiring specialized microscopes and ex vivo tissue processing. Here we used ligand-dependent protein stabilization to create a new cAMP sensor. This sensor allows specific and sensitive detection of cAMP in living zebrafish embryos, which may enable new understanding of the functions of cAMP in living vertebrates.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping