PUBLICATION
The cellular architecture and molecular determinants of the zebrafish fusogenic synapse
- Authors
- Luo, Z., Shi, J., Pandey, P., Ruan, Z.R., Sevdali, M., Bu, Y., Lu, Y., Du, S., Chen, E.H.
- ID
- ZDB-PUB-220622-9
- Date
- 2022
- Source
- Developmental Cell 57(13): 1582-1597.e6 (Journal)
- Registered Authors
- Chen, Elizabeth, Du, Shao Jun (Jim), Luo, Zhou, Shi, Jun
- Keywords
- Jam2a/Jamb, Jam3b/Jamc, Myomaker, Myomixer, WASP and WAVE, actin cytoskeleton, cell-cell fusion, invasive membrane protrusions, myoblast fusion, zebrafish muscle development
- MeSH Terms
-
- Actins*/metabolism
- Animals
- Cell Fusion
- Drosophila/metabolism
- Membrane Proteins/genetics
- Membrane Proteins/metabolism
- Muscle Development
- Muscle Proteins
- Synapses/metabolism
- Zebrafish*/genetics
- Zebrafish*/metabolism
- PubMed
- 35709765 Full text @ Dev. Cell
Citation
Luo, Z., Shi, J., Pandey, P., Ruan, Z.R., Sevdali, M., Bu, Y., Lu, Y., Du, S., Chen, E.H. (2022) The cellular architecture and molecular determinants of the zebrafish fusogenic synapse. Developmental Cell. 57(13):1582-1597.e6.
Abstract
Myoblast fusion is an indispensable process in skeletal muscle development and regeneration. Studies in Drosophila led to the discovery of the asymmetric fusogenic synapse, in which one cell invades its fusion partner with actin-propelled membrane protrusions to promote fusion. However, the timing and sites of vertebrate myoblast fusion remain elusive. Here, we show that fusion between zebrafish fast muscle cells is mediated by an F-actin-enriched invasive structure. Two cell adhesion molecules, Jam2a and Jam3b, are associated with the actin structure, with Jam2a being the major organizer. The Arp2/3 actin nucleation-promoting factors, WAVE and WASP-but not the bipartite fusogenic proteins, Myomaker or Myomixer-promote the formation of the invasive structure. Moreover, the convergence of fusogen-containing microdomains and the invasive protrusions is a prerequisite for cell membrane fusion. Thus, our study provides unprecedented insights into the cellular architecture and molecular determinants of the asymmetric fusogenic synapse in an intact vertebrate animal.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping